Anti-CHCHD5抗体[4F3] (ab119428)

概述

  • 产品名称Anti-CHCHD5抗体[4F3]
  • 描述
    小鼠单克隆抗体[4F3] to CHCHD5
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-110 of Human CHCHD5 produced in E.coli (NP_115685) .

  • 阳性对照
    • HEK293T cell lysate transfected with pCMV6-ENTRY CHCHD5 cDNA; Human breast, endometrium, endometrium adenocarcinoma, bladder carcinoma and tonsil tissues; HEK293T cells transfected with a CHCHD5 overexpress plasmid; Hela and Jurkat cells
  • 常规说明Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号4F3
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab119428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200. Predicted molecular weight: 12 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 序列相似性Contains 1 CHCH domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110027L01Rik antibody
    • AW045710 antibody
    • C2orf9 antibody
    • CHCH5_HUMAN antibody
    • CHCHD5 antibody
    • chromosome 2 open reading frame antibody
    • Coiled coil helix coiled coil helix domain containing 5 antibody
    • Coiled-coil-helix-coiled-coil-helix domain-containing protein 5 antibody
    • FLJ39671 antibody
    • MGC11104 antibody
    • RP24-388N11.2 antibody
    see all

Anti-CHCHD5 antibody [4F3] 图像

  • All lanes : Anti-CHCHD5 antibody [4F3] (ab119428) at 1/200 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CHCHD5 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 12 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203980)
  • ab119428, at 1/150 dilution, staining CHCHD5 in paraffin-embedded Human breast tissue by Immunohistochemistry.
  • ab119428, at 1/150 dilution, staining CHCHD5 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab119428, at 1/150 dilution, staining CHCHD5 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab119428, at 1/150 dilution, staining CHCHD5 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
  • ab119428, at 1/150 dilution, staining CHCHD5 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
  • HEK293T cells transfected with either a CHCHD5 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained using ab119428 at 1/100 dilution and then analyzed by flow cytometry.
  • Flow cytometric analysis of CHCD5 in Hela cells using ab119428 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).
  • Flow cytometric analysis of CHCHD5 in Jurkat cells, using ab119428 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).

Anti-CHCHD5 antibody [4F3] (ab119428)参考文献

ab119428 has not yet been referenced specifically in any publications.

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