antigen sequence, corresponding to amino acids 493-620 of Human CHADL.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class IV subfamily.
Contains 19 LRR (leucine-rich) repeats.
Contains 2 LRRCT domains.
Contains 2 LRRNT domains.
Secreted > extracellular space > extracellular matrix.
Information by UniProt
- AY100452 antibody
- CHADL antibody
- CHADL_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-CHADL antibody (ab121228)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab121228 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHADL antibody (ab121228)
ab121228 at 1/10- dilution staining CHADL in Paraffin-embedded Human tonsil tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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