概述

  • 产品名称
  • 描述
    兔多克隆抗体to CHAC1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human CHAC1 aa 188-237 (C terminal).
    Sequence:

    TPQNPGYLGPAPEEAIATQILACRGFSGHNLEYLLRLADFMQLCGPQAQD

  • 阳性对照
    • 721_B cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution (PubMed:23070364). Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (PubMed:19109178). Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed:22445366).
  • 序列相似性
    Belongs to the gamma-glutamylcyclotransferase family. ChaC subfamily.
  • 细胞定位
    Cytoplasm, cytosol. Golgi apparatus, trans-Golgi network.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Blocks Notch protein antibody
    • Botch antibody
    • Cation transport regulator like protein 1 antibody
    • Cation transport regulator-like protein 1 antibody
    • ChaC antibody
    • ChaC cation transport regulator homolog 1 antibody
    • ChaC cation transport regulator like 1 antibody
    • chac1 antibody
    • CHAC1_HUMAN antibody
    • Gamma GCT acting on glutathione homolog 1 antibody
    • Gamma-GCG 1 antibody
    • Glutathione-specific gamma-glutamylcyclotransferase 1 antibody
    see all

图片

  • Anti-CHAC1 antibody (ab76386) at 1 µg/ml + 721_B cell lysate at 10 µg

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    Gel concentration: 12%

实验方案

文献

This product has been referenced in:

See all 2 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Mouse Purified protein (Brain tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
30 µg
Treatment
Hypoxia ischemia-injured tissue 0, 1, 6, & 24h and mouse liver lysa
Specification
Brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Jan 19 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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