Anti-CH25H抗体(ab76478)

概述

  • 产品名称Anti-CH25H抗体
    参阅全部 CH25H 一抗
  • 描述
    小鼠单克隆抗体to CH25H
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Rat, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 142-247 of Human CH25H (AAH17843) with a 26 kDa enzyme tag.

  • 阳性对照
    • Recombinant CH25H fragment

性能

应用

Our Abpromise guarantee covers the use of ab76478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 13 kDa plus 26 kDa tag).
    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Function: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Similarity: Belongs to the sterol desaturase family. PTM: N-glycosylated.
    • 细胞定位Endoplasmic reticulum membrane
    • 数据库链接
    • 别名
      • C25H antibody
      • Ch25h antibody
      • CH25H_HUMAN antibody
      • Cholesterol 25 hydroxylase antibody
      • Cholesterol 25 monooxygenase antibody
      • Cholesterol 25-hydroxylase antibody
      • Cholesterol 25-monooxygenase antibody
      • EC 1.14.99.38 antibody
      • EC=1.14.99.38 antibody
      • h25OH antibody
      see all

    Anti-CH25H antibody 图像

    • Anti-CH25H antibody (ab76478) at 5 µg/ml + recombinant fragment of CH25H (plus 26 kDa tag) at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 13 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    Anti-CH25H antibody (ab76478)参考文献

    ab76478 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Rat Tissue lysate - whole (Liver tissue)
    Loading amount 50 µg
    Specification Liver tissue
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

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    提交于 Feb 08 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"