概述

  • 产品名称
  • 描述
    山羊多克隆抗体to CGRP
  • 特异性
    Antibody is thought to react with whole molecule (1-37) and 23-37 fragment (C term). Specifically visualises CGRP in brain cells.
  • 经测试应用
    适用于: IHC-FoFr, ELISA, IHC-P, IHC-Frmore details
    不适用于: IHC-R
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Rat CGRP (C terminal).
    Sequence:

    VKDNFVPTNVGSEAF

性能

应用

Our Abpromise guarantee covers the use of ab36001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent dilution. PubMed: 19805319
ELISA 1/500 - 1/2500.
IHC-P 1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/200.
  • 应用说明
    Is unsuitable for IHC-R.
  • 靶标

    • 相关性
      CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
    • 细胞定位
      Endoplasmic reticulum and Secreted
    • 数据库链接
    • 别名
      • Calcitonin/calcitonin related polypeptide alpha antibody
      • Alpha type CGRP antibody
      • Beta type CGRP antibody
      • CALC1 antibody
      • CALC2 antibody
      • CALCA antibody
      • CALCB antibody
      • Calcitonin 1 antibody
      • Calcitonin 2 antibody
      • Calcitonin gene related peptide I antibody
      • Calcitonin gene related peptide II antibody
      • Calcitonin related polypeptide alpha antibody
      • Calcitonin related polypeptide beta antibody
      • CGRP antibody
      • CGRP I antibody
      • CGRP II antibody
      • CGRP1 antibody
      • CGRP2 antibody
      • CT antibody
      • Katacalcin antibody
      • KC antibody
      see all

    图片

    • IHC-P image of CGRP staining on rat spinal cord sections using ab36001 (1/3000). The sections were deparafinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. The primary antibody was used 21°C for 2 hours.

      See Abreview

    • ab36001 staining mouse colon tissue sections by IHC-Fr.  Sections were PFA fixed and blocked in 5% serum for 30 minutes at 24°C prior to incubating with ab36001, diluted 1/200 in PBS + 0.2% Triton X-100, for 18 hours at 4°C.  The secondary antibody was an Alexa Fluor® 546 conjugated, diluted 1/200.

      See Abreview

    文献

    This product has been referenced in:
    • Ouyang H  et al. Ulinastatin attenuates neuropathic pain induced by L5-VRT via the calcineurin/IL-10 pathway. Mol Pain 12:N/A (2016). Read more (PubMed: 27175013) »
    • Wang H  et al. Increasing expression of substance P and calcitonin gene-related peptide in synovial tissue and fluid contribute to the progress of arthritis in developmental dysplasia of the hip. Arthritis Res Ther 17:4 (2015). IHC . Read more (PubMed: 25578529) »

    See all 27 Publications for this product

    客户评价及客户问答

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Dorsal Root Ganglia)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Dorsal Root Ganglia
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Jean-Marc Guedon

    Verified customer

    提交于 Jun 03 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Feb 25 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Sep 06 2012

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain, spinal cord, muscle w/nerve)
    Specification
    brain, spinal cord, muscle w/nerve
    Fixative
    formalin, 10% neutral buffered
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH6, microwave 2x, 5 min on high
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 18 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Colon)
    Specification
    Colon
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 08 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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