The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. PubMed: 19805319
1/500 - 1/2500.
1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Is unsuitable for IHC-R.
CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
Calcitonin/calcitonin related polypeptide alpha antibody
Alpha type CGRP antibody
Beta type CGRP antibody
Calcitonin 1 antibody
Calcitonin 2 antibody
Calcitonin gene related peptide I antibody
Calcitonin gene related peptide II antibody
Calcitonin related polypeptide alpha antibody
Calcitonin related polypeptide beta antibody
CGRP I antibody
CGRP II antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab36001)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom
IHC-P image of CGRP staining on rat spinal cord sections using ab36001 (1/3000). The sections were deparafinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. The primary antibody was used 21°C for 2 hours.
Immunohistochemistry (Frozen sections) - Anti-CGRP antibody (ab36001)This image is courtesy of an anonymous Abreview
ab36001 staining mouse colon tissue sections by IHC-Fr. Sections were PFA fixed and blocked in 5% serum for 30 minutes at 24°C prior to incubating with ab36001, diluted 1/200 in PBS + 0.2% Triton X-100, for 18 hours at 4°C. The secondary antibody was an Alexa Fluor® 546 conjugated, diluted 1/200.
Wei D et al. Orai1 and Orai3 Mediate Store-Operated Calcium Entry Contributing to Neuronal Excitability in Dorsal Root Ganglion Neurons. Front Cell Neurosci11:400 (2017).
Read more (PubMed: 29311831) »
Ouyang H et al. Ulinastatin attenuates neuropathic pain induced by L5-VRT via the calcineurin/IL-10 pathway. Mol Pain12:N/A (2016).
Read more (PubMed: 27175013) »