概述

  • 产品名称Anti-CGRP抗体[4901]
    参阅全部 CGRP 一抗
  • 描述
    小鼠单克隆抗体[4901] to CGRP
  • 特异性ab81887 is specific for Calcitonin Gene Related Peptide. It also recognizes rat beta CGRP and human alpha CGRP (10 fold less sensitivity compared to rat alpha CGRP).
  • 经测试应用适用于: Neutralising, ICC/IF, Flow Cyt, IHC-Frmore details
    不适用于: WB
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Human
    预测可用于: all Mammals
  • 免疫原

    Rat alpha-CGRP

  • 阳性对照
    • Dorsal root ganglia, pancreas and gut.
  • 常规说明


    This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • 纯度Protein G purified
  • Primary antibody说明This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue.
  • 克隆单克隆
  • 克隆编号4901
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Neutralising Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration. PubMed: 24430801

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/1000.
  • 应用说明Is unsuitable for WB.
  • 靶标

    Anti-CGRP antibody [4901] 图像

    • ab81887 staining rat DRG sections by IHC-Fr. The sections were fixed with 4% paraformaldehyde, permeabilised in 0.3% PBS-Tween and blocked for non-specific protein-protein interactions with 10% donkey's serum for 1h at 24°C. Staining with ab81887 at a 1/1000 dilution in PBS-Triton (0.3%) with 10% donkey's serum and 0.02% azide was performed for 24h at 4°C. A donkey anti-mouse Alexa568 polyclonal antibody at 1/1000 was used as the secondary antibody.

      See Abreview

    • Immunofluorescent staining of CGRP (red) in cryopreserved DRG neurons grown on coverslips using ab81887 at 1/50 dilution. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5.
    • ab81887 staining CGRP in Mouse dorsal root ganglion tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 2% BSA and 2% goat serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/300 in blocking solution) for 12 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (2 mg/ml) was used as the secondary antibody.

      See Abreview

    Anti-CGRP antibody [4901] (ab81887)参考文献

    This product has been referenced in:
    • Mei F  et al. Accelerated remyelination during inflammatory demyelination prevents axonal loss and improves functional recovery. Elife 5:N/A (2016). IHC-FoFr ; Mouse . Read more (PubMed: 27671734) »
    • Li Y  et al. Toll-like receptor 4 enhancement of non-NMDA synaptic currents increases dentate excitability after brain injury. Neurobiol Dis 74:240-53 (2015). Read more (PubMed: 25497689) »

    See all 11 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (Nervus Suralis peripheral neurons)
    Permeabilization Yes - Triton X-100
    Specification Nervus Suralis peripheral neurons
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
    Fixative Acetone
    Username

    Mr. Thomas Klein

    Verified customer

    提交于 Sep 11 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Pig Cell (Dorsal root ganglion neuron)
    Permeabilization Yes - Triton X
    Specification Dorsal root ganglion neuron
    Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 37°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 May 05 2015

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Sample Rat Tissue sections (Dorsal root ganglian)
    Specification Dorsal root ganglian
    Permeabilization Yes - 0.3% TritonX-100
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Mar 16 2015

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    Thank you very much for contacting us with your questions.

    Our antibodies are intended for in vitro use, and so we do not have a protocol for in vivo use or information about the half-life of the antibody. The concentration of ab81887 is lot-...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (DRG neurons)
    Specification DRG neurons
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.3% TritonX in 0.1% PBS
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    提交于 Mar 21 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (dorsal root ganglion)
    Specification dorsal root ganglion
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step 2% BSA and 2% goat serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
    Username

    有作 岩崎

    Verified customer

    提交于 Feb 04 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"