概述

  • 产品名称cGMP Direct Immunoassay试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • 检测类型Quantitative
  • 灵敏度
    > 0.008 µM
  • 范围
    0.008 µM - 2 µM
  • 产品概述

    Abcam's cGMP Direct Immunoassay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples. The kit utilizes the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450nm. The intensity of OD450nm is inversely proportional to the concentration of cGMP in samples. The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04-10 pmol/well (0.008-2 µM) cGMP samples.
    Visit our FAQs page for tips and troubleshooting.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Cyclic guanosine monophosphate (cGMP) is a cyclic nucleotide derived from guanosine triphosphate (GTP). It serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG).
  • 别名
    • Cyclic GMP
    • Cyclic guanosine monophosphate
    • Guanosine 3’ 5’ cyclic monophosphate

应用

Our Abpromise guarantee covers the use of ab65356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

cGMP Direct Immunoassay Kit 图像

  • O.D.'s of human urine measured at various dilutions (+/- SD)

  • Standard curve showing mean of duplicates (+/-SD) with background readings subtracted

实验方案

cGMP Direct Immunoassay Kit (ab65356)参考文献

This product has been referenced in:
  • Kondo K  et al. H2S protects against pressure overload-induced heart failure via upregulation of endothelial nitric oxide synthase. Circulation 127:1116-27 (2013). Read more (PubMed: 23393010) »
  • Kang YH  et al. Vasodilatory Effects of Cinnamic Acid via the Nitric Oxide-cGMP-PKG Pathway in Rat Thoracic Aorta. Phytother Res N/A:N/A (2012). Read more (PubMed: 22517576) »

See all 3 Publications for this product

Product Wall

You can use a speedvac to dry down samples.



After adding the acetylating reagent mix, incubate at RT for 10 mins, then put the tubes in a laminar airflow hood or simply air-dry. 5ul will dry-up fairly quickly. For low amounts of cGMP, add 50-100ul of the assay buffer to dried tubes and...

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Thank you for contacting us.
On page 7 the section b of assay protocol states "for suspension cells collect by centrifugation. Add 1 ml of 0.1M HCl for every 35 cm2 of surface area (e.g., 10 cm plate at 70% confluency is ˜110 cm2, so use ˜3.1 ml)....

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The protcol booklet has been updated with missing information. Please check.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Thank you for your recent telephone enquiry.

I can confirm that the Bradford assay can be used for protein quantifation with this kit.

The BCA protein assay is not compatible with the acidic buffer in the samples you would have pr...

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Yes, the supernatant from this extraction can be stored at -80°C in aliquots for later analysis.

We don’t have a recommended protocol for collection of blood for this kit. I would just suggest storing the blood on ice before processing it for serum/plasma collection.


Hope this information has been helpful for you. Please let ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"