概述

  • 产品名称Anti-CG18321抗体
  • 描述
    小鼠多克隆抗体to CG18321
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 57-156 of Fruit fly (Drosophila melanogaster) CG18321. Target sequence used to make the antibody: AETVASTTNS DTHRTRNNRK PNKPQEHSQQ VGAHKSGSRK LVVAENGGAL SSQLPQPNHK QGHKNTDKQQ RGGGSKQQRG HSQHHGGNRK GPKAATPENG.

  • 常规说明

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 30 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 相关性CG18321 (miple2) is a member of the MK/PTN family of proteins. Drosophila Miple1 and miple2 are secreted proteins which are expressed in spatially restricted, nonoverlapping patterns during embryogenesis. CG18321 is strongly expressed in the developing midgut endoderm. The exact molecular functions and biological processes in which it is involved are not known. Mutations have been isolated which are viable and fertile.
  • 数据库链接
    • 别名
      • Miple2 antibody
      • RE13914p antibody

    Anti-CG18321 antibody 图像

    • All lanes : Anti-CG18321 antibody (ab52682) at 1/1000 dilution

      Lane 1 : Total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
      Lane 2 : Total protein extract from E coli with 50ng to 500ng of the antigen (tagged antigen fusion protein).

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM, HRP conjugated, at 1/5000 dilution

      Predicted band size : 30 kDa

    Anti-CG18321 antibody (ab52682)参考文献

    ab52682 has not yet been referenced specifically in any publications.

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