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CFSE is a versatile tool for the fluorescent intracellular labeling of live cells. Labeled cells can be assayed using flow cytometry and fluorescent microscopy. The dye is long lasting and well retained within labeled cells. The provided CFSE is sufficient for ~1000 assays.
CFSE (5(6)-Carboxyfluorescein N-hydroxysuccinimidyl ester) is a cell permeant, non-fluorescent pro-dye. Intracellular esterases in live cells cleave the acetate groups which results in the green fluorescent molecule carboxyfluorescein that is now membrane impermeant. The succinimidyl ester group reacts indiscriminately with intracellular free amines to generate covalent dye-protein conjugates. The result is live cells with an intracellular fluorescent label. At appropriate concentrations CFSE is non-toxic to cells and the fluorescence is retained after formaldehyde and alcohol fixation. CFSE labeled cells can be detected with any instrument or filter set compatible with fluroescein detection: Excitation(max)=492nm, Emission(max)=517nm.
|10mM CFSE (in DMSO)||1 x 100µl|
Our Abpromise guarantee covers the use of ab113853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|