概述

  • 产品名称
  • 描述
    兔多克隆抗体to CFP10
  • 特异性
    ab45074 recognises native and E coli expressed recombinant CFP10.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    Reacts with Mycobacterium tuberculosis and Mycobacterium bovis. Not yet tested in other species.
  • 免疫原

    Full length protein (Mycobacterium tuberculosis)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    Purity: > 95% (by SDS-PAGE)
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab45074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: Use at a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 12.3 kDa.
    Application note: Reducing conditions.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      CFP10 (10 kDa culture filtrate protein) is a low molecular weight protein isolated from Mycobacterium tuberculosis culture filtrates. CFP10 and the 6 kDa early secretory antigen of T cells (ESAT6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Bacille Calmette Guerin (BCG). CFP10 is one of a small number of secreted M. tuberculosis proteins now identified, that appear to play key roles in tuberculosis pathogenesis and in the stimulation of immunity.
    • 细胞定位
      Secreted
    • 别名
      • 10 kDa culture filtrate antigen cfp10 antibody
      • Culture filtrate protein 10 antibody
      • ESAT 6 like protein esxB antibody
      • esxB antibody
      • lhp antibody
      • mtsA10 antibody
      • Secreted antigenic protein MTSA 10 antibody
      • tuberculosis CFP10 antibody
      see all

    实验方案

    文献

    This product has been referenced in:
    • Sharma G  et al. The interaction of mycobacterial protein Rv2966c with host chromatin is mediated through non-CpG methylation and histone H3/H4 binding. Nucleic Acids Res 43:3922-37 (2015). WB ; Human . Read more (PubMed: 25824946) »
    • Ramsdell TL  et al. Linked domain architectures allow for specialization of function in the FtsK/SpoIIIE ATPases of ESX secretion systems. J Mol Biol 427:1119-32 (2015). Read more (PubMed: 24979678) »

    See all 2 Publications for this product

    客户评价及客户问答

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Mycobacterium tuberculosis Cell lysate - other (M. tuberculosis culture filtrate)
    Total protein in input
    1000 µg
    Specification
    M. tuberculosis culture filtrate
    Immuno-precipitation step
    Protein G
    Username

    Abcam user community

    Verified customer

    提交于 Jul 11 2012

    Application
    Western blot
    Sample
    Mycobacterium tuberculosis Purified protein (M. tuberculosis culture filtrate)
    Loading amount
    10 µg
    Specification
    M. tuberculosis culture filtrate
    Gel Running Conditions
    Reduced Denaturing (15% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 27 2012

    Thank you for your interest in Abcam.

    1) Indeed, I can confirm that all relevant antibodies (CFP10: ab45074, ab64753, ab64754; ESAT6: ab26246, ab45073; Ag85B: ab43019) have been generated against the full-length proteins. The exact binding se...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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