Anti-CETP抗体(ab51771)

概述

  • 产品名称Anti-CETP抗体
    参阅全部 CETP 一抗
  • 描述
    兔多克隆抗体to CETP
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Hamster, Non Human Primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human CETP.

    (Peptide available as ab71389.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa, Jurkat, SK N BE, Y79 and HepG2.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab51771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 55 kDa).

靶标

  • 功能Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 疾病相关Defects in CETP are a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
    Defects in CETP are the cause of cholesteryl ester transfer protein deficiency (CETP deficiency) [MIM:607322]. This is an autosomal dominant condition associated with increased HDL cholesterol levels.
  • 序列相似性Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BPIFF antibody
    • CETP antibody
    • CETP_HUMAN antibody
    • Cholesteryl ester transfer protein antibody
    • Cholesteryl ester transfer protein plasma antibody
    • HDLCQ10 antibody
    • Lipid transfer protein I antibody
    see all

Anti-CETP antibody 图像

  • All lanes : Anti-CETP antibody (ab51771) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 4 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ICC/IF image of ab51771 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51771, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab51771 staining CETP in Human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51771, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CETP antibody (ab51771)参考文献

ab51771 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.
The protease inhibitors are added in the lysis buffer so when you do centrifugation there is no need of adding protease inhibitors in supernatant again.
Supernatant I mean the media when the cells are pelleted out. Th...

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Could you provide the details of distributor form whom this product was purchased? Are they official Abcam distributor? Please note, if they are not official then Abpromise guarantee will not be applied to...

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Could you clarify the problem? Are you concerned that antibody shows multiple bands? If yes could you answer the following questions;
- What is the order number and how the antibody was stored?
- Have you added...

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This particular product ab51771 does not contain BSA when the antibody concentration is ≥1.00 mg/ml, which is the case for the lot we currently have in stock. BSA is added in the storage buffer of ab51771 when t...

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Thank you for contacting us. We do not have any free samples of the liver tissue that was used to validate this antibody for IHC, but if you are interested in purchasing sections of other liver tissue, we have sections of paraffin-embedded human liv...

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Thank you for contacting us. The conditions for the IHC validation are described briefly in the caption of the image on the online datasheet, which shows a stain of a formalin-fixed, paraffin-embedded human liver section. The section was pre-trea...

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