Anti-Ceruloplasmin抗体(ab110449)

概述

  • 产品名称
    Anti-Ceruloplasmin抗体
    参阅全部 Ceruloplasmin 一抗
  • 描述
    兔多克隆抗体to Ceruloplasmin
  • 经测试应用
    适用于: IHC-FoFr, WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Pig
  • 免疫原

    Synthetic peptide within Human Ceruloplasmin aa 350-450 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    (Peptide available as ab111803)

  • 阳性对照
    • This antibody gave a positive signal in Human plasma total protein lysate as well as Human liver tissue lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab110449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 148 kDa (predicted molecular weight: 122 kDa).
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • 组织特异性
    Expressed by the liver and secreted in plasma.
  • 疾病相关
    Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • 序列相似性
    Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CERU_HUMAN antibody
    • Ceruloplasmin antibody
    • CP 2 antibody
    • CP antibody
    • CP2 antibody
    • Ferroxidase antibody
    see all

图片

  • ab110449 staining ceruloplasmin in astroglial cells from rat by ICC/IF (Immunocytochemistry/immunofluorescence).  Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in 0.1% PBS and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody for 4 hours at 24°C. An undiluted Alexa Fluor® 488-conjugated Donkey polyclonal was used as the secondary antibody.

     

  • Adult rat was perfused with 4% PFA in 0.1M PBS. Fluorescent staining was performed in duodenum sections with anti-Ceruloplasmin (ab110449) primary and Alexa 488 conjugated secondary antibody. This antibody stained gut enterocytes. Nucleus stained with Hoechst at high power magnification.

    See Abreview

  • All lanes : Anti-Ceruloplasmin antibody (ab110449) at 1 µg/ml

    Lane 1 : Human Plasma Total Protein Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 122 kDa
    Observed band size : 148 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa,75 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • Anti-Ceruloplasmin antibody (ab110449) at 1 µg/ml + Natural Human Ceruloplasmin protein (ab77930) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 122 kDa


    Exposure time : 30 seconds

文献

ab110449 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your comment.

Before offering a testing discount for new species, our Scientific Team need to check the sequence homology to see if the cross-reactivity is expected or not.

If the homology is higher than 95%, then ther...

Read More
Application
Western blot
Sample
Pig Tissue lysate - whole (endometrial tissue lysate)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
30 µg
Specification
endometrial tissue lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jun 26 2015

Thank you for the reply.

I have checked the sequence homology between human and pig (Sus scrofa) and it is only 87%. This means that the probability of this antibody to recognize pig (porcine) Ceruloplasmin is low. Please advise your customer...

Read More

Gracias po contactarnos.

En principio no habría ningún problema para conjugar estos anticuerpos. Lo que si es necesario tener en cuenta es que el kit de conjugación sea compatible con los buffers y aditivos en los que conserva el anticuerpo. ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Duodenum)
Specification
Duodenum
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Sep 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Astro-glia)
Specification
Astro-glia
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Sep 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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