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Synthetic peptide within Human Ceruloplasmin aa 350-450 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
(Peptide available as
Our Abpromise guarantee covers the use of ab110449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 148 kDa (predicted molecular weight: 122 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
ab110449 staining ceruloplasmin in astroglial cells from rat by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in 0.1% PBS and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody for 4 hours at 24°C. An undiluted Alexa Fluor® 488-conjugated Donkey polyclonal was used as the secondary antibody.
ab110449 has not yet been referenced specifically in any publications.