概述

  • 产品名称Anti-CERKL抗体
    参阅全部 CERKL 一抗
  • 描述
    兔多克隆抗体to CERKL
  • 特异性This antibody detects endogenous levels of total CERKL protein.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthesized peptide derived from the internal region of human CERKL.

  • 阳性对照
    • Extracts from A549 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: 1/20000.
    WB: 1/500 - 1/1000. Detects bands of approximately 63, 40 and 48 kDa (predicted molecular weight: 63 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.
    • 组织特异性Isoform 1 and isoform 2 are expressed in adult retina, liver and pancreas as well as in fetal brain, lung and kidney. Isoform 3 is expressed in adult retina as well as in fetal lung and liver. Isoform 4 is expressed in adult retina, lung and kidney as well as in fetal lung and liver. Moderately expressed in retina, kidney, lung, testis, trachea, and pancreas. Weakly expressed in brain, placenta and liver.
    • 疾病相关Defects in CERKL are the cause of retinitis pigmentosa type 26 (RP26) [MIM:608380]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP26 inheritance is autosomal recessive.
    • 序列相似性Contains 1 DAGKc domain.
    • 发展阶段Expressed in fetal lung, kidney and brain.
    • 翻译后修饰Phosphorylated on serine residues.
    • 细胞定位Cytoplasm. Nucleus > nucleolus. Enriched in nucleoli. May shuttle between nucleus and cytoplasm. Isoform 5 is not enriched in the nucleoli and Cytoplasm. Nucleus > nucleolus. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Ceramide kinase like protein antibody
      • Ceramide kinase-like protein antibody
      • CERKL antibody
      • CERKL_HUMAN antibody
      • Retinitis pigmentosa 26 (autosomal recessive) antibody
      • RP26 antibody
      see all

    Anti-CERKL antibody 图像

    • All lanes : Anti-CERKL antibody (ab66007) at 1/500 dilution

      Lane 1 : Extracts from A549 cells
      Lane 2 : Extracts from A549 cells, plus 5µg immunizing peptide

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 63 kDa
      Observed band size : 63 kDa
      Additional bands at : 40 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    Anti-CERKL antibody (ab66007)参考文献

    This product has been referenced in:
    • Jelcick AS  et al. Genetic variations strongly influence phenotypic outcome in the mouse retina. PLoS One 6:e21858 (2011). WB ; Mouse . Read more (PubMed: 21779340) »

    See 1 Publication for this product

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    Cet anticorps n’a pas encore été testé chez la souris et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires.<...

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