概述

  • 产品名称Anti-CERK抗体
    参阅全部 CERK 一抗
  • 描述
    兔多克隆抗体to CERK
  • 经测试应用适用于: WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Hamster, Human, Chinese Hamster
  • 免疫原

    Synthetic peptide corresponding to Human CERK aa 487-516 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • Mouse heart tissue lysate. A2058 cell lysate. IF/ICC: SKNSH cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明This antibody is purified through a protein G column, eluted with both high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab38011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.

靶标

  • 功能Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.
  • 组织特异性High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung.
  • 序列相似性Contains 1 DAGKc domain.
  • 细胞定位Cytoplasm. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acylsphingosine kinase antibody
    • Ceramide kinase antibody
    • Cerk antibody
    • CERK1_HUMAN antibody
    • dA59H18.2 antibody
    • dA59H18.3 antibody
    • hCERK antibody
    • KIAA1646 antibody
    • Lipid kinase 4 antibody
    • Lipid kinase LK4 antibody
    • LK4 antibody
    see all

Anti-CERK antibody 图像

  • ICC/IF image of ab38011 stained SKNSH cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38011, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-CERK antibody (ab38011) at 1/100 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : A2058 cell lysate


    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Mouse heart tissue lysate (Lane 1), A2058 cell lysate (Lane 2).

Anti-CERK antibody (ab38011)参考文献

This product has been referenced in:
  • Gubern A  et al. JNK and Ceramide Kinase Govern the Biogenesis of Lipid Droplets through Activation of Group IVA Phospholipase A2. J Biol Chem 284:32359-69 (2009). WB ; Chinese Hamster . Read more (PubMed: 19778898) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Hamster Cell lysate - whole cell (CHO-K1 cells)
Loading amount 20 µg
Specification CHO-K1 cells
Treatment CHO-K1 cells treated with three siRNA duplexes designed to knock down CERK expression
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Jan 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"