Anti-Ceramide glucosyltransferase抗体(ab124296)

概述

  • 产品名称Anti-Ceramide glucosyltransferase抗体
    参阅全部 Ceramide glucosyltransferase 一抗
  • 描述
    兔多克隆抗体to Ceramide glucosyltransferase
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Chicken, Cow, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Ceramide glucosyltransferase.

  • 阳性对照
    • This antibody gave a positive signal in MCF7 and A375 whole cell lysates, and in the following tissue lysates: Human Brain; Mouse Brain; Rat Brain; Mouse Spleen; Rat Spleen.

性能

应用

Our Abpromise guarantee covers the use of ab124296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).

靶标

Anti-Ceramide glucosyltransferase antibody 图像

  • All lanes : Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Spleen (Mouse) Tissue Lysate
    Lane 5 : Spleen (Rat) Tissue Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : A375 (Human melanoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 12 kDa,36 kDa,68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-Ceramide glucosyltransferase antibody (ab124296)参考文献

ab124296 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab124296.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"