存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).
功能 Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase".
组织特异性 Found in all tissues examined.
通路 Lipid metabolism; sphingolipid metabolism.
序列相似性 Belongs to the glycosyltransferase 2 family.
细胞定位 Golgi apparatus membrane.
Information by UniProt
Ceramide glucosyltransferase antibody
Anti-Ceramide glucosyltransferase antibody 图像
Western blot - Anti-Ceramide glucosyltransferase antibody (ab124296)
All lanes : Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/ml Lane 1 : Human brain tissue lysate - total protein ( ab29466) Lane 2 : Brain (Mouse) Tissue Lysate Lane 3 : Brain (Rat) Tissue Lysate Lane 4 : Spleen (Mouse) Tissue Lysate Lane 5 : Spleen (Rat) Tissue Lysate Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : A375 (Human melanoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 45 kDa Observed band size : 48 kDa ( why is the actual band size different from the predicted?) Additional bands at : 12 kDa,36 kDa,68 kDa. We are unsure as to the identity of these extra bands. Exposure time : 30 seconds
Anti-Ceramide glucosyltransferase antibody (ab124296)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"