兔多克隆抗体to Ceramide glucosyltransferase
Mouse, Rat, Human
Rabbit, Chicken, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Ceramide glucosyltransferase.
This antibody gave a positive signal in MCF7 and A375 whole cell lysates, and in the following tissue lysates: Human Brain; Mouse Brain; Rat Brain; Mouse Spleen; Rat Spleen.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).
Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase".
Found in all tissues examined.
Lipid metabolism; sphingolipid metabolism.
Belongs to the glycosyltransferase 2 family.
Golgi apparatus membrane.
Information by UniProt
Ceramide glucosyltransferase antibody
Western blot - Anti-Ceramide glucosyltransferase antibody (ab124296)
All lanes :
Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/ml
Lane 1 :
Human brain tissue lysate - total protein (
Lane 2 :
Brain (Mouse) Tissue Lysate
Lane 3 :
Brain (Rat) Tissue Lysate
Lane 4 :
Spleen (Mouse) Tissue Lysate
Lane 5 :
Spleen (Rat) Tissue Lysate
Lane 6 :
MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 :
A375 (Human melanoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
48 kDa (
why is the actual band size different from the predicted?
Additional bands at :
12 kDa,36 kDa,68 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
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