Anti-Ceramide glucosyltransferase抗体(ab124296)

概述

  • 产品名称
    Anti-Ceramide glucosyltransferase抗体
    参阅全部 Ceramide glucosyltransferase 一抗
  • 描述
    兔多克隆抗体to Ceramide glucosyltransferase
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Chicken, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Ceramide glucosyltransferase.

  • 阳性对照
    • This antibody gave a positive signal in MCF7 and A375 whole cell lysates, and in the following tissue lysates: Human Brain; Mouse Brain; Rat Brain; Mouse Spleen; Rat Spleen.

性能

应用

Our Abpromise guarantee covers the use of ab124296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).

靶标

图片

  • All lanes : Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Spleen (Mouse) Tissue Lysate
    Lane 5 : Spleen (Rat) Tissue Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : A375 (Human melanoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 12 kDa, 36 kDa, 68 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124296 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

实验方案

文献

This product has been referenced in:
  • Sun R  et al. Lowered humidity produces human epidermal equivalents with enhanced barrier properties. Tissue Eng Part C Methods 21:15-22 (2015). Read more (PubMed: 24803151) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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