A synthetic peptide corresponding to a region between residues 625 and 675 of human CEP97. NP_078824.2
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 97 kDa.
Use at 2-5 µg/mg of lysate.
Acts as a key negative regulator of ciliogenesis in collaboration with CCP110 by capping the mother centriole thereby preventing cilia formation. Required for recruitment of CCP110 to the centrosome.
Contains 1 IQ domain.
Contains 8 LRR (leucine-rich) repeats. Contains 1 LRRCT domain.
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Information by UniProt
Western blot - Anti-CEP97 antibody (ab86007)
All lanes : Anti-CEP97 antibody (ab86007) at 1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Predicted band size: 97 kDa Detection by chemiluminescence with exposure time of 3 minutes.
Immunoprecipitation - Anti-CEP97 antibody (ab86007)
ab86007 used at 3µg/mg lysate, and at 1µg/ml for western blot. 1mg HeLa whole cell lysate used for immunoprecipitation, 20% loaded (Lane 1). Control IgG (Lane 2).
Detection by chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"