Synthetic peptide corresponding to a region between residues 639 and 689 of human CEP78 (CAI40237.1)
- HeLa and 293T whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
CEP78 belongs to the CEP78 family and contains 3 LRR (leucine rich) repeats.
- 5730599I05Rik antibody
- C9orf81 antibody
- Centrosomal protein 78 antibody
Western blot - CEP78 antibody (ab86188)
All lanes : Anti-CEP78 antibody (ab86188) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 76 kDa
Observed band size : 76 kDa
Exposure time : 3 minutes
Immunoprecipitation - CEP78 antibody (ab86188)
Detection of human CEP78 in Immunoprecipitates of HeLa whole cell lysate (1 mg klysate for IP, 20% of IP loaded) using ab86188 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"