概述

  • 产品名称Anti-CEP76抗体
    参阅全部 CEP76 一抗
  • 描述
    兔多克隆抗体to CEP76
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Rhesus monkey, Opossum, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 30 of Human CEP76 (NP_079175.2).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab86613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
IP Use at 10 µg/mg of lysate.

靶标

  • 功能Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication.
  • 序列相似性Belongs to the CEP76 family.
  • 发展阶段Expressed at low level in G1 and is induced in S and G2 phase, during which point centrioles have already commenced duplication (at protein level).
  • 细胞定位Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Does not localize along the ciliary axoneme.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C18orf9 antibody
    • Centrosomal protein 76kDa antibody
    • Centrosomal protein of 76 kDa antibody
    • CEP 76 antibody
    • Cep76 antibody
    • CEP76_HUMAN antibody
    • Chromosome 18 open reading frame 9 antibody
    • FLJ12542 antibody
    • HsT1705 antibody
    see all

Anti-CEP76 antibody 图像

  • All lanes : Anti-CEP76 antibody (ab86613) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 76 kDa
    Observed band size : 69 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 115 kDa,305 kDa. We are unsure as to the identity of these extra bands.
  • Western Blot detection of CEP76 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86613 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with exposure time of 30 seconds

Anti-CEP76 antibody (ab86613)参考文献

ab86613 has not yet been referenced specifically in any publications.

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