产品名称 Anti-CEP76抗体 参阅全部 CEP76 一抗
WB, IP more details
Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Rhesus monkey, Opossum, Orangutan
Synthetic peptide corresponding to a region between residues 1 and 30 of Human CEP76 (NP_079175.2).
HeLa and 293T whole cell lysates.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
Use at 10 µg/mg of lysate.
功能 Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication.
序列相似性 Belongs to the CEP76 family.
发展阶段 Expressed at low level in G1 and is induced in S and G2 phase, during which point centrioles have already commenced duplication (at protein level).
细胞定位 Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Does not localize along the ciliary axoneme.
Information by UniProt
Centrosomal protein 76kDa antibody
Centrosomal protein of 76 kDa antibody
Anti-CEP76 antibody 图像
Western blot - CEP76 antibody (ab86613)
All lanes : Anti-CEP76 antibody (ab86613) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Predicted band size : 76 kDa Observed band size : 69 kDa ( why is the actual band size different from the predicted?) Additional bands at : 115 kDa,305 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - CEP76 antibody (ab86613)
Western Blot detection of CEP76 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86613 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 30 seconds
Anti-CEP76 antibody (ab86613)参考文献
has not yet been referenced specifically in any publications.
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