概述

  • 产品名称Anti-CEP72抗体
    参阅全部 CEP72 一抗
  • 描述
    兔多克隆抗体to CEP72
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residues 150 and 200 of human CEP72 protein (NP_060610.2)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab75254 was affinity purified using an epitope specific to CEP72 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 72 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in the recruitment of key centrosomal proteins to the centrosome. Provides centrosomal microtubule-nucleation activity on the gamma-tubulin ring complexes (gamma-TuRCs) and has critical roles in forming a focused bipolar spindle, which is needed for proper tension generation between sister chromatids. Required for localization of KIZ/PLK1S1, AKAP9 and gamma-tubulin ring complexes (gamma-TuRCs).
    • 序列相似性Belongs to the CEP72 family.
      Contains 3 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
    • 细胞定位Cytoplasm > cytoskeleton > centrosome. Localizes to the centrosome and centrosome-surrounding particles throughout the cell cycle. These particles disappear after microtubules are depolymerized using nocodazole, suggesting that CEP72-associating particles localize in a microtubule-dependent manner.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Centrosomal protein 72kDa antibody
      • Centrosomal protein of 72 kDa antibody
      • Cep72 antibody
      • CEP72_HUMAN antibody
      • FLJ10565 antibody
      • MGC5307 antibody
      • RGD1566336 antibody
      see all

    Anti-CEP72 antibody 图像

    • All lanes : Anti-CEP72 antibody (ab75254) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 72 kDa
      Observed band size : 72 kDa


      Exposure time : 3 minutes
    • Detection of CEP72 by Western Blot of Immunprecipitate.
      ab75254, at 1µg/ml, staining CEP72 in HeLa whole cell lysate immunoprecipitated using ab75254 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.

    Anti-CEP72 antibody (ab75254)参考文献

    ab75254 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab75254.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"