Synthetic peptide corresponding to a region within the internal sequence amino acids 350-400 (PTNVSPNCPP AEATALPFSG PREPSLKQWP SRVPQKQGGM GLASWSQLAS T) of Human CEP68 (NP_055962.2).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
This antibody was affinity purified using an epitope specific to CEP68 immobilized on a solid support
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
Use at 5-15 µg/mg of lysate.
Cytoplasm > cytoskeleton > centrosome.
Information by UniProt
Centrosomal protein 68kDa antibody
Centrosomal protein of 68 kDa antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP68 antibody (ab91460)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling CEP68 with ab91460 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-CEP68 antibody (ab91460)
ab91460 at 1 µg/ml detecting CEP68 in HeLa whole cell lysate by WB following IP. Immunoprecipitation utilised ab91460 at 10 µg/mg of lysate (lane 2), CEP68 was also immunoprecipitated by an antibody to an upstream epitope of CEP68 (lane 1) but not by control IgG (lane 3). 1 mg of lysate was used for IP and 20% of IP was loaded.
Detection utilised ECL with a 30 second exposure.
Western blot - Anti-CEP68 antibody (ab91460)
All lanes : Anti-CEP68 antibody (ab91460) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Developed using the ECL technique. Predicted band size: 81 kDa Exposure time: 3 minutes
has not yet been referenced specifically in any publications.
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