Anti-CEP68抗体(ab91460)

概述

  • 产品名称Anti-CEP68抗体
    参阅全部 CEP68 一抗
  • 描述
    兔多克隆抗体to CEP68
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 350-400 (PTNVSPNCPP AEATALPFSG PREPSLKQWP SRVPQKQGGM GLASWSQLAS T) of Human CEP68 (NP_055962.2).

  • 阳性对照
    • HeLa whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was affinity purified using an epitope specific to CEP68 immobilized on a solid support
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
IP Use at 5-15 µg/mg of lysate.

靶标

Anti-CEP68 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling CEP68 with ab91460 at 1/1000 (1µg/ml). Detection: DAB.
  • ab91460 at 1 µg/ml detecting CEP68 in HeLa whole cell lysate by WB following IP. Immunoprecipitation utilised ab91460 at 10 µg/mg of lysate (lane 2), CEP68 was also immunoprecipitated by an antibody to an upstream epitope of CEP68 (lane 1) but not by control IgG (lane 3). 1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 30 second exposure.
  • All lanes : Anti-CEP68 antibody (ab91460) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 81 kDa


    Exposure time : 3 minutes

Anti-CEP68 antibody (ab91460)参考文献

ab91460 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (CHO)
Specification CHO
Permeabilization No
Fixative Methanol
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提交于 Dec 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"