概述

  • 产品名称Anti-CEP55抗体
    参阅全部 CEP55 一抗
  • 描述
    兔多克隆抗体to CEP55
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide selected from the N-terminal region of Human CEP55 conjugated to KLH (NP_001120654.1).

  • 阳性对照
    • MDA-MB231 cell line lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab91469 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 54 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.
    • 组织特异性Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.
    • 翻译后修饰There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.
    • 细胞定位Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > centrosome. Cleavage furrow. Midbody. Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C10orf3 antibody
      • cancer/testis antigen 111 antibody
      • Centrosomal protein 55kDa antibody
      • Centrosomal protein of 55 kDa antibody
      • CEP 55 antibody
      • Cep55 antibody
      • CEP55_HUMAN antibody
      • CT111 antibody
      • FLJ10540 antibody
      • Up regulated in colon cancer 6 antibody
      • Up-regulated in colon cancer 6 antibody
      • URCC 6 antibody
      • URCC6 antibody
      see all

    Anti-CEP55 antibody 图像

    • Anti-CEP55 antibody (ab91469) at 2 µg/ml + MDA-MB231 cell line lysate at 35 µg

      Predicted band size : 54 kDa

    Anti-CEP55 antibody (ab91469)参考文献

    ab91469 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"