Anti-CEP170抗体(ab72505)
Key features and details
- Rabbit polyclonal to CEP170
- Suitable for: ICC, WB, IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-CEP170抗体
参阅全部 CEP170 一抗 -
描述
兔多克隆抗体to CEP170 -
宿主
Rabbit -
经测试应用
适用于: ICC, WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Cow, Chimpanzee -
免疫原
Synthetic peptide corresponding to a region between residue 1534 and the C-teriminus (residue 1584) of human CEP170 (NP_055627.2)
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab72505 was affinity purified using an epitope specific to CEP170 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72505于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
Use at an assay dependent concentration.
|
|
WB |
Use a concentration of 0.04 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 176 kDa).
|
|
IP |
Use at 6 µg/mg of lysate.
|
说明 |
---|
ICC
Use at an assay dependent concentration. |
WB
Use a concentration of 0.04 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 176 kDa). |
IP
Use at 6 µg/mg of lysate. |
靶标
-
相关性
CEP170, centrosomal protein 170kDa, plays a role in microtubule organization. During interphase, the encoded protein localizes to the sub-distal appendages of mature centrioles, which are microtubule-based structures thought to help organize centrosomes. During mitosis, the protein associates with spindle microtubules near the centrosomes. -
细胞定位
Centrosome, centriole. Cytoplasm, cytoskeleton. Associated with the mature mother centriole. Associated with spindle microtubules during mitosis. -
数据库链接
- Entrez Gene: 9859 Human
- SwissProt: Q5SW79 Human
-
别名
- XRCC5 binding protein antibody
- 4933426L22Rik antibody
- A330004A13Rik antibody
see all
图片
-
All lanes : Anti-CEP170 antibody (ab72505) at 0.04 µg/ml
Lane 1 : HEK293T whole cell lysate
Lane 2 : A-549 whole cell lysate
Lane 3 : K-562 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 176 kDa -
CEP170 was immunoprecipitated from HeLa whole cell lysate with ab72505. Western blot was performed on the immunoprecipitate using ab72505 at 0.04µg/ml.
Lane 1 : HeLa whole cell lysate
Lane 2 : Control IgG
Chemiluminescence with an exposure time of 10 seconds.
-
Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling CEP170 with ab72505 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (7)
ab72505 被引用在 7 文献中.
- Tischer T et al. The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assembly. J Cell Sci 135:N/A (2022). PubMed: 34878135
- Chong WM et al. Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages. Elife 9:N/A (2020). PubMed: 32242819
- Song K et al. A SEPT1-based scaffold is required for Golgi integrity and function. J Cell Sci 132:N/A (2019). PubMed: 30709970
- Lin JR et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362
- Bärenz F et al. Ccdc61 controls centrosomal localization of Cep170 and is required for spindle assembly and symmetry. Mol Biol Cell N/A:mbcE18020115 (2018). PubMed: 30354798
- Shumilov A et al. Epstein-Barr virus particles induce centrosome amplification and chromosomal instability. Nat Commun 8:14257 (2017). PubMed: 28186092
- Yu TW et al. Mutations in WDR62, encoding a centrosome-associated protein, cause microcephaly with simplified gyri and abnormal cortical architecture. Nat Genet 42:1015-20 (2010). ICC/IF ; Human . PubMed: 20890278