概述

  • 产品名称Anti-CEP135抗体
    参阅全部 CEP135 一抗
  • 描述
    兔多克隆抗体to CEP135
  • 特异性ab75005 detects endogenous levels of total CEP135 protein.
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    synthesized peptide derived from C-terminal region of human CEP135.

  • 阳性对照
    • extracts from COLO cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB 1/500 - 1/1000. Predicted molecular weight: 134 kDa.
ELISA 1/40000.

靶标

  • 功能Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.
  • 疾病相关Microcephaly 8, primary, autosomal recessive
  • 序列相似性Belongs to the CEP135/TSGA10 family.
  • 细胞定位Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • centrosomal protein 135 kDa antibody
    • centrosomal protein 135kDa antibody
    • Centrosomal protein 4 antibody
    • Centrosomal protein of 135 kDa antibody
    • centrosome protein 4 antibody
    • centrosome protein cep135 antibody
    • Cep135 antibody
    • CEP4 antibody
    • CP135_HUMAN antibody
    see all

Anti-CEP135 antibody 图像

  • All lanes : Anti-CEP135 antibody (ab75005) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 134 kDa
    Observed band size : 134 kDa

Anti-CEP135 antibody (ab75005)参考文献

This product has been referenced in:
  • Shiratsuchi G  et al. RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 34:97-114 (2015). Human . Read more (PubMed: 25385835) »
  • Sawant DB  et al. Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes centrin 2 function. Mol Biol Cell 26:3741-53 (2015). Read more (PubMed: 26354417) »

See all 5 Publications for this product

Product Wall

Thanks for your reply.
It does seem that the vial of ab75005 you received is not working as expected. I am very sorry for the trouble. If you have received this antibody within the past 6 months or so I would be happy to offer a free replacement o...

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Thank you for your enquiry. I am sorry to hear this vial of ab75005 is giving you trouble.
To ensure that the cell lysates are not degraded, I was wondering if you had successfully blotted these same cell lysates with any other antibodies?
Th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3)
Specification NIH3T3
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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提交于 Jul 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"