Synthetic peptide corresponding to a region within amino acids 1044-1094 of Human CEP128 (NP_689659.2).
293T and HeLa whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 128 kDa.
Use at 2-10 µg/mg of lysate.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Associates with the mother centriole.
Information by UniProt
Western blot - Anti-CEP128 antibody (ab118797)
All lanes : Anti-CEP128 antibody (ab118797) at 1 µg/ml Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 128 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-CEP128 antibody (ab118797)
Detection of CEP128 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118797 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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