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Synthetic peptide conjugated to KLH, corresponding to amino acids 157-172 of Human Centrin 1, with N-terminally added lysine. This sequence is highly conserved in centrin 1 of many species, e.g. mouse, drosophila, and Xenopus. It is also found in human and mouse centrin 2, but is not found in centrin 3.
Our Abpromise guarantee covers the use of ab11257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 21 kDa. 1/2000, this concentration is recommended using a nuclear extract of the human epitheloid carcinoma HeLa cell line. Predicted molecular weight: 21 kDa.|
|ICC/IF||1/200. PubMed: 17299043
Suggested working dilution of 1/200 (see PMID: 17299043).
Methanol fixation may be preferable for this antibody based on publications and customer feedback.
ab11257 staining Centrin 1 in mouse embryonic fibroblast cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with Ethanol (-200C) and blocking with 3% BSA/PBS was done for 30 minutes at 220C. Samples were incubated with primary antibody (1/500) in in 3% BSA /0.1 % saponin/PBS for 1 hour at RT. An undiluted Alexa Fluor®546-conjugated donkey polyclonal to goat IgG (H&L) was used at dilution at 1/500 as secondary antibody. In the image, Green staining represents gamma tubulin, red - centrin.
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