概述

  • 产品名称Anti-Centlein抗体
    参阅全部 Centlein 一抗
  • 描述
    兔多克隆抗体to Centlein
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: LIEARKEVEV SQSKYNALSL QLSNKQTELI QKDMDITLVR KELQELQNLY KQNSTHTAQQ AELIQQLQVL NMDTQKVLRN QEDVHTAESI S corresponding to amino acids 289-379 of Human Centlein.

  • 阳性对照
    • Human liver tissue; Human liver lysate; Human cell line U-251 MG

性能

应用

Our Abpromise guarantee covers the use of ab122356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.

靶标

  • 细胞定位Cytoplasm > cytoskeleton > centrosome > centriole. Colocalizes with gamma-tubulin during interphase and mitosis. Appears to associated with the mother centriole during G1 phase and with daughter centrioles towards G1/S phase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA340N12.1 antibody
    • C9orf101 antibody
    • C9orf39 antibody
    • Centlein antibody
    • Centlein centrosomal protein antibody
    • Centrosomal protein antibody
    • Cntln antibody
    • CNTLN_HUMAN antibody
    • FLJ20276 antibody
    • RP11-340N12.1 antibody
    see all

Anti-Centlein antibody 图像

  • ab122356, at 1/60 dilution, staining Centlein in paraffin-embedded Human liver tissue by Immunohistochemistry.
  • All lanes : Anti-Centlein antibody (ab122356) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate
  • ab122356, at 4 µg/ml, staining Centlein in Human cell line U-251 MG by Immunofluorescence. Antibody staining is shown in green.

Anti-Centlein antibody (ab122356)参考文献

ab122356 has not yet been referenced specifically in any publications.

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