The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.
Cytoplasm > cytoskeleton > centrosome > centriole. Colocalizes with gamma-tubulin during interphase and mitosis. Appears to associated with the mother centriole during G1 phase and with daughter centrioles towards G1/S phase.