概述

  • 产品名称Anti-CENTB1抗体
    参阅全部 CENTB1 一抗
  • 描述
    小鼠多克隆抗体to CENTB1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human CENTB1, amino acids 1-740 (NP_055531.1)

  • 阳性对照
    • Human spleen tissue lysate, K562 cell lysate and CENTB1 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab89766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 82 kDa.

靶标

  • 功能GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.
  • 组织特异性Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
  • 序列相似性Contains 3 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 BAR domain.
    Contains 1 PH domain.
  • 结构域PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.
  • 翻译后修饰Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACAP 1 antibody
    • ACAP1 antibody
    • ACAP1_HUMAN antibody
    • Arf GAP with coiled coil ANK repeat and PH domains 1 antibody
    • Arf GAP with coiled coil, ankyrin repeat, and pleckstrin homology domains 1 antibody
    • Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 antibody
    • ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 antibody
    • CENT B1 antibody
    • Centaurin beta 1 antibody
    • Centaurin-beta-1 antibody
    • CENTB 1 antibody
    • Cnt b1 antibody
    • Cnt-b1 antibody
    • Cntb1 antibody
    • KIAA0050 antibody
    see all

Anti-CENTB1 antibody 图像

  • Anti-CENTB1 antibody (ab89766) at 1 µg/ml + Human spleen tissue lysate at 50 µg

    Predicted band size : 82 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
  • Anti-CENTB1 antibody (ab89766) at 1 µg/ml + K562 cell lysate at 50 µg

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
    Additional bands at : 35 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-CENTB1 antibody (ab89766) at 1 µg/ml

    Lane 1 : CENTB1 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 82 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

Anti-CENTB1 antibody (ab89766)参考文献

ab89766 has not yet been referenced specifically in any publications.

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