Mouse, Rat, Cow, Dog
Synthetic peptide: EKLSRRSHDLHTL, corresponding to C terminal amino acids 728-740 of Human CENTB1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris saline. pH 7.3
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 80-90 kDa (predicted molecular weight: 85 kDa).Can be blocked with
Human CENTB1 peptide (ab23280).
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.
Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
Contains 3 ANK repeats.
Contains 1 Arf-GAP domain. Contains 1 BAR domain. Contains 1 PH domain.
PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.
Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Information by UniProt
ACAP 1 antibody
Western blot - Anti-CENTB1 antibody (ab15903)
Anti-CENTB1 antibody (ab15903) at 1 µg/ml + Jurkat cell lysate at 35 µg
Predicted band size:
Additional bands at:
80 kDa. We are unsure as to the identity of these extra bands.
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"