Anti-Centaurin gamma 1抗体(ab87643)

概述

  • 产品名称
    Anti-Centaurin gamma 1抗体
    参阅全部 Centaurin gamma 1 一抗
  • 描述
    山羊多克隆抗体to Centaurin gamma 1
  • 特异性
    This antibody is expected to recognize isoform PIKE-S (NP_055585.1)
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Centaurin gamma 1 aa 46-60.
    Sequence:

    C-QASLDSIREAVINSQ


    Database link: NP_055585.1

  • 阳性对照
    • Human brain (Amygdala & Cerebral Cortex)

性能

应用

Our Abpromise guarantee covers the use of ab87643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).

靶标

  • 功能
    GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
  • 组织特异性
    Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart.
  • 序列相似性
    Belongs to the centaurin gamma-like family.
    Contains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
  • 结构域
    G domain binds GTP and has GTPase activity.
    Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
    Although both PH domains of isoforms 1 and 2 bind phospholipids, they differently regulate subcellular location. PH domain of isoform 1 directs the protein to the nucleus, but PH domain of isoform 2 directs it to the cytosol. PH domain of isoform 2 is required for binding to AP-1.
  • 翻译后修饰
    Isoform PIKE-A is phosphorylated at Tyr-682 and Tyr-774 by FYN, preventing its apoptotic cleavage.
  • 细胞定位
    Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AGAP-2 antibody
    • Agap2 antibody
    • Agap2 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody
    • AGAP2_HUMAN antibody
    • ANK repeat and PH domains 2 antibody
    • Arf GAP with GTP binding protein like antibody
    • Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2 antibody
    • Arf GAP with GTP-binding protein-like, ankyrin repeat and pleckstrin homology domains 2 antibody
    • Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 antibody
    • ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody
    • centaurin gamma1 antibody
    • Centaurin-gamma-1 antibody
    • Centg1 antibody
    • cnt g1 antibody
    • Cnt-g1 antibody
    • Cnt2 antibody
    • FLJ16430 antibody
    • GGAP2 antibody
    • GTP binding and GTPase activating protein 2 antibody
    • GTP-binding and GTPase activating protein 2 antibody
    • GTP-binding and GTPase-activating protein 2 antibody
    • KIAA0167 antibody
    • mKIAA0167 antibody
    • Nuclear GTPase PIKE antibody
    • Phosphatidylinositol 3-kinase enhancer antibody
    • Phosphoinositide 3 kinase enhancer antibody
    • PI3K ENHANCER antibody
    • PIKE antibody
    see all

图片

  • Anti-Centaurin gamma 1 antibody (ab87643) at 0.3 µg/ml + Human amygdala lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 91 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

文献

ab87643 has not yet been referenced specifically in any publications.

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