Anti-PIKE抗体(ab21271)
Key features and details
- Rabbit polyclonal to PIKE
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-PIKE抗体
参阅全部 PIKE 一抗 -
描述
兔多克隆抗体to PIKE -
宿主
Rabbit -
特异性
Recognises PIKE: also detect both PIKE-L and PIKE-A isoforms. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Human PIKE (C terminal).
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阳性对照
- Mouse brain cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified by ion exchange chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21271于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 150, 80 kDa (predicted molecular weight: 90 kDa).
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说明 |
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IHC-P
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 150, 80 kDa (predicted molecular weight: 90 kDa). |
靶标
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功能
GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. -
组织特异性
Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart. -
序列相似性
Belongs to the centaurin gamma-like family.
Contains 2 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 PH domain. -
结构域
G domain binds GTP and has GTPase activity.
Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
Although both PH domains of isoforms 1 and 2 bind phospholipids, they differently regulate subcellular location. PH domain of isoform 1 directs the protein to the nucleus, but PH domain of isoform 2 directs it to the cytosol. PH domain of isoform 2 is required for binding to AP-1. -
翻译后修饰
Isoform PIKE-A is phosphorylated at Tyr-682 and Tyr-774 by FYN, preventing its apoptotic cleavage. -
细胞定位
Cytoplasm and Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 105242480 Mouse
- Entrez Gene: 216439 Mouse
- SwissProt: Q3UHD9 Mouse
- Unigene: 336761 Mouse
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别名
- AGAP-2 antibody
- Agap2 antibody
- Agap2 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody
see all
图片
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Lane 1 : Anti-PIKE antibody (ab21271) at 0.5 µg/ml
Lane 2 : Anti-PIKE antibody (ab21271) at 1 µg/ml
Lane 3 : Anti-PIKE antibody (ab21271) at 2 µg/ml
All lanes : Mouse brain cell lysate
Predicted band size: 90 kDa
Ab21271 at concentrations of 0.5 (lane A), 1 (lane B) and 2µ g/ml (lane C), staining PIKE-L and PIKE-A in mouse brain cell lysates by Western blot. -
ab21271 at 10µg/ml staining PIKE in mouse brain tissue by IHC
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab21271 尚未被引用在任何文献中。