Anti-Centaurin gamma 1抗体(ab21271)


  • 产品名称
    Anti-Centaurin gamma 1抗体
    参阅全部 Centaurin gamma 1 一抗
  • 描述
    兔多克隆抗体to Centaurin gamma 1
  • 宿主
  • 特异性
    Recognises Centaurin gamma 1: also detect both PIKE-L and PIKE-A isoforms.
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    This antibody was raised against a synthetic peptide corresponding to 15 amino acids near the C-terminus of human PIKE.

  • 阳性对照
    • Mouse brain cell lysates.



Our Abpromise guarantee covers the use of ab21271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 150, 80 kDa (predicted molecular weight: 90 kDa).


  • 功能
    GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
  • 组织特异性
    Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart.
  • 序列相似性
    Belongs to the centaurin gamma-like family.
    Contains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
  • 结构域
    G domain binds GTP and has GTPase activity.
    Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
    Although both PH domains of isoforms 1 and 2 bind phospholipids, they differently regulate subcellular location. PH domain of isoform 1 directs the protein to the nucleus, but PH domain of isoform 2 directs it to the cytosol. PH domain of isoform 2 is required for binding to AP-1.
  • 翻译后修饰
    Isoform PIKE-A is phosphorylated at Tyr-682 and Tyr-774 by FYN, preventing its apoptotic cleavage.
  • 细胞定位
    Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AGAP-2 antibody
    • Agap2 antibody
    • Agap2 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody
    • AGAP2_HUMAN antibody
    • ANK repeat and PH domains 2 antibody
    • Arf GAP with GTP binding protein like antibody
    • Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2 antibody
    • Arf GAP with GTP-binding protein-like, ankyrin repeat and pleckstrin homology domains 2 antibody
    • Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 antibody
    • ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody
    • centaurin gamma1 antibody
    • Centaurin-gamma-1 antibody
    • Centg1 antibody
    • cnt g1 antibody
    • Cnt-g1 antibody
    • Cnt2 antibody
    • FLJ16430 antibody
    • GGAP2 antibody
    • GTP binding and GTPase activating protein 2 antibody
    • GTP-binding and GTPase activating protein 2 antibody
    • GTP-binding and GTPase-activating protein 2 antibody
    • KIAA0167 antibody
    • mKIAA0167 antibody
    • Nuclear GTPase PIKE antibody
    • Phosphatidylinositol 3-kinase enhancer antibody
    • Phosphoinositide 3 kinase enhancer antibody
    • PI3K ENHANCER antibody
    • PIKE antibody
    see all


  • Lane 1 : Anti-Centaurin gamma 1 antibody (ab21271) at 0.5 µg/ml
    Lane 2 : Anti-Centaurin gamma 1 antibody (ab21271) at 1 µg/ml
    Lane 3 : Anti-Centaurin gamma 1 antibody (ab21271) at 2 µg/ml

    All lanes : Mouse brain cell lysate

    Predicted band size: 90 kDa

    Ab21271 at concentrations of 0.5 (lane A), 1 (lane B) and 2 µg/ml (lane C), staining PIKE-L and PIKE-A in mouse brain cell lysates by Western blot.  
  • ab21271 at 10µg/ml staining Centaurin gamma 1 in mouse brain tissue by IHC


ab21271 has not yet been referenced specifically in any publications.


Thank you for your interest.

I have conducted a search for you and found 7 antibodies against this target (PIKE, GGAP2 AGAP2, Centaurin). Here are some which have been tested in Western blot application and Immunostaining, it seems that none ...

Read More