概述

  • 产品名称Anti-CENPT抗体
    参阅全部 CENPT 一抗
  • 描述
    兔多克隆抗体to CENPT
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 480 - 520 (LEMVEKCLDK YFQHLCDDLE VFAAHAGRKT VKPEDLELLM) of Human CENPT (NP_079358.3)

  • 阳性对照
    • Whole cell lysate from HeLa cells or 293T cells.

性能

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应用

Our Abpromise guarantee covers the use of ab86595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
IP Use at 10 µg/mg of lysate.

靶标

  • 功能Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENP-T is one of the basic inner kinetochore proteins with most further proteins binding downstream, suggesting a fundamental role of CENP-T in kinetochore function.
  • 序列相似性Belongs to the CENPT family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C16orf56 antibody
    • CENP T antibody
    • CENP-T antibody
    • Cenpt antibody
    • CENPT_HUMAN antibody
    • Centromere protein T antibody
    • Chromosome 16 open reading frame 56 antibody
    • ICEN22 antibody
    • Interphase centromere complex protein 22 antibody
    see all

Anti-CENPT antibody 图像

  • All lanes : Anti-CENPT antibody (ab86595) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 60 kDa


    Exposure time : 3 minutes
  • 1mg whole cell lysate from Hela cells was immunoprecipited using 10ug ab86594.
    20% of IP was loaded per lane, and probed with ab86549 at 1ug/ml (lane 1) or with a control IgG (lane 2).
    Detection: hemiluminescence with an exposure time of 30 seconds.

Anti-CENPT antibody (ab86595)参考文献

ab86595 has not yet been referenced specifically in any publications.

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