概述

  • 产品名称Anti-CENPJ抗体
    参阅全部 CENPJ 一抗
  • 描述
    兔多克隆抗体to CENPJ
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human CENPJ.

    (Peptide available as ab28894.)

  • 阳性对照
    • This antibody gave a positive signal in Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate

性能

应用

Our Abpromise guarantee covers the use of ab26052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 149 kDa (predicted molecular weight: 153 kDa).

靶标

  • 功能Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome.
  • 疾病相关Defects in CENPJ are the cause of microcephaly primary type 6 (MCPH6) [MIM:608393]. A disorder defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits.
    Defects in CENPJ are the cause of Seckel syndrome type 4 (SCKL4) [MIM:613676]. SCKL4 is a rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation.
  • 序列相似性Belongs to the TCP10 family.
  • 翻译后修饰Phosphorylation at Ser-589 and Ser-595 by PLK2 is required for procentriole formation and centriole elongation. Phosphorylation by PLK2 oscillates during the cell cycle: it increases at G1/S transition and decreases during the exit from mitosis. Phosphorylation at Ser-595 is also mediated by PLK4 but is not a critical step in PLK4 function in procentriole assembly.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Localized within the center of microtubule asters. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CENP-J antibody
    • CENPJ antibody
    • CENPJ_HUMAN antibody
    • Centromere protein J antibody
    • Centrosomal P4.1-associated protein antibody
    • CPAP antibody
    • LAG-3-associated protein antibody
    • LAP antibody
    • LIP1 antibody
    • LYST-interacting protein 1 antibody
    • MCPH6 antibody
    see all

Anti-CENPJ antibody 图像

  • Anti-CENPJ antibody (ab26052) at 1 µg/ml + Jurkat (Human) Nuclear Lysate - CD3 activated (ab14845) at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 153 kDa
    Observed band size : 149 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

Anti-CENPJ antibody (ab26052)参考文献

ab26052 has not yet been referenced specifically in any publications.

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