概述

  • 产品名称Anti-CENPI抗体
    参阅全部 CENPI 一抗
  • 描述
    兔多克隆抗体to CENPI
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 50-100 of Human CENPI (NP_006724.2).

  • 阳性对照
    • HeLa, 293T and Jurkat whole cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab118796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 87 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 相关性CENPI is a constitutive protein of the knetochore that shares limited similarity with Mis6 of S. pombe. The kinetochore is a macromolecular complex located at the centromere of chromosomes and provides essential functions for chromosome segregration. CENPI is required for the localization of CENPF and the checkpoint proteins MAD1 and MAD2 to the kinetochore.
  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
  • 别名
    • CENP I antibody
    • centromere protein I antibody
    • Follicle simulating hormone primary response protein antibody
    • FSH primary response 1 antibody
    • FSH primary response protein 1 antibody
    • FSH primary response, rat, homolog of, 1 antibody
    • FSHP1 antibody
    • FSHPRH1 antibody
    • interphase centromere complex protein 19 antibody
    • Leucine rich primary response gene 1 antibody
    • Leucine rich primary response protein 1 antibody
    • LRPR1 antibody
    • Mis6 antibody
    see all

Anti-CENPI antibody 图像

  • All lanes : Anti-CENPI antibody (ab118796) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 87 kDa


    Exposure time : 30 seconds
  • Detection of CENPI in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118796 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-CENPI antibody (ab118796)参考文献

ab118796 has not yet been referenced specifically in any publications.

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