Anti-CENPH抗体(ab88593)
Key features and details
- Mouse polyclonal to CENPH
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CENPH抗体
参阅全部 CENPH 一抗 -
描述
小鼠多克隆抗体to CENPH -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein within Human CENPH aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- HeLa cell lysate. Raw 264.7 cell lysate. NIH/3T3 cell lysate. Lysate from transfected 293T cells. HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88593于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. -
序列相似性
Belongs to the centromere protein H family. -
细胞定位
Nucleus. Chromosome > centromere > kinetochore. Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC1 during both interphase and metaphase. - Information by UniProt
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数据库链接
- Entrez Gene: 64946 Human
- Entrez Gene: 26886 Mouse
- Omim: 605607 Human
- SwissProt: Q9H3R5 Human
- SwissProt: Q9QYM8 Mouse
- Unigene: 631967 Human
- Unigene: 273502 Mouse
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别名
- CENP H antibody
- CENP-H antibody
- CENPH antibody
see all
图片
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Anti-CENPH antibody (ab88593) at 5 µg/ml + HeLa cell lysate at 25 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Anti-CENPH antibody (ab88593) at 5 µg/ml + Raw 264.7 cell lysate at 25 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Anti-CENPH antibody (ab88593) at 5 µg/ml + NIH/3T3 cell lysate at 25 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Additional bands at: ~19 kDa, ~60 kDa, ~70 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-CENPH antibody (ab88593) at 5 µg/ml
Lane 1 : cell lysate from CENPH transfected 293T cells
Lane 2 : cell lysate from non transfected 293T cells
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 28 kDa -
ab88593 at 10 µg/ml detecting CENPH in HeLa cells by immunofluorescence.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab88593 被引用在 2 文献中.
- Hu L et al. Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment. Nucleic Acids Res 47:468-479 (2019). PubMed: 30407575
- Zhang T et al. CenpH regulates meiotic G2/M transition by modulating the APC/CCdh1-cyclin B1 pathway in oocytes. Development 144:305-312 (2017). PubMed: 27993978