概述

  • 产品名称Anti-CENPH抗体
    参阅全部 CENPH 一抗
  • 描述
    小鼠单克隆抗体to CENPH
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human CENPH protein (AAH12024) with a 26 kDa tag.

  • 阳性对照
    • CENPH transfected 293T cell lysate; immunogen

性能

应用

Our Abpromise guarantee covers the use of ab77207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
  • 序列相似性Belongs to the centromere protein H family.
  • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC1 during both interphase and metaphase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CENP H antibody
    • CENP-H antibody
    • CENPH antibody
    • CENPH_HUMAN antibody
    • Centromere protein H antibody
    • ICEN35 antibody
    • Interphase centromere complex protein 35 antibody
    • Kinetochore protein CENP H antibody
    • NNF1 antibody
    • NNF1, MIND kinetochore complex component, homolog antibody
    • PMF1 antibody
    see all

Anti-CENPH antibody 图像

  • Anti-CENPH antibody (ab77207) at 1/500 dilution + recombinant tagged human CENPH protein (the immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 28 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CENPH antibody (ab77207) at 1/500 dilution

    Lane 1 : CENPH transfected 293T cell lysate
    Lane 2 : Non transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 28 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Anti-CENPH antibody (ab77207)参考文献

ab77207 has not yet been referenced specifically in any publications.

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