概述

  • 产品名称Anti-CENPF抗体
    参阅全部 CENPF 一抗
  • 描述
    兔多克隆抗体to CENPF
  • 经测试应用适用于: ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1800-1850 of Human CENPF

  • 阳性对照
    • HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab93236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
  • 应用说明ICC/IF: 1/100 - 1/500.
    Acetone fixation should be used. Formaldehyde or methanol fixation is not recommended.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.
    • 疾病相关Stromme syndrome
    • 序列相似性Belongs to the centromere protein F family.
    • 发展阶段Gradually accumulates during the cell cycle, reaching peak levels in G2 and M phase, and is rapidly degraded upon completion of mitosis.
    • 翻译后修饰Hyperphosphorylated during mitosis.
    • 细胞定位Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AH antigen antibody
      • Cell cycle dependent 350K nuclear protein antibody
      • CENF antibody
      • CENP F antibody
      • CENP-F antibody
      • CENPF antibody
      • CENPF kinetochore protein antibody
      • CENPF_HUMAN antibody
      • Centromere protein F 350/400ka antibody
      • Centromere protein F antibody
      • Hcp 1 antibody
      • Hcp1 antibody
      • Kinetochore protein CENP F antibody
      • Kinetochore protein CENPF antibody
      • Mitosin antibody
      • PRO1779 antibody
      see all

    Anti-CENPF antibody 图像

    • Detection of Human CENPF in Acetone fixed asynchronous HeLa cells by Immunocytochemistry using ab93236 at a dilution of 1/250. Detection: FITC conjugated anti-rabbit IgG used at a dilution of 1/100.
    • Detection of Human CENPF in Acetone fixed asynchronous HeLa cells by Immunocytochemistry using ab93236 at a dilution of 1/250. Detection: FITC conjugated anti-rabbit IgG used at a dilution of 1/100.

    Anti-CENPF antibody (ab93236)参考文献

    ab93236 has not yet been referenced specifically in any publications.

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