Anti-CENPF抗体(ab84697)

概述

  • 产品名称Anti-CENPF抗体
    参阅全部 CENPF 一抗
  • 描述
    兔多克隆抗体to CENPF
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide:

    QAEIQIKEES KTAVEMLQN Q LKELNEAV AA LCGDQEI MKA TEQSLD PPIE EEHQL RNSIE

    , corresponding to amino acids 2350-2400 of Human CENPF

  • 阳性对照
    • Human controls: Breast Carcinoma, Colon Carcinoma, Ewing Sarcoma, Stomach Adenocarcinoma

性能

应用

Our Abpromise guarantee covers the use of ab84697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.
  • 疾病相关Stromme syndrome
  • 序列相似性Belongs to the centromere protein F family.
  • 发展阶段Gradually accumulates during the cell cycle, reaching peak levels in G2 and M phase, and is rapidly degraded upon completion of mitosis.
  • 翻译后修饰Hyperphosphorylated during mitosis.
  • 细胞定位Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AH antigen antibody
    • Cell cycle dependent 350K nuclear protein antibody
    • CENF antibody
    • CENP F antibody
    • CENP-F antibody
    • CENPF antibody
    • CENPF kinetochore protein antibody
    • CENPF_HUMAN antibody
    • Centromere protein F 350/400ka antibody
    • Centromere protein F antibody
    • Hcp 1 antibody
    • Hcp1 antibody
    • Kinetochore protein CENP F antibody
    • Kinetochore protein CENPF antibody
    • Mitosin antibody
    • PRO1779 antibody
    see all

Anti-CENPF antibody 图像

  • ab84697 staining CENPF in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG H&L polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

    See Abreview

  • ab84697, at a dilution of 1/100, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
    Detection: Red fluorescent goat anti-rabbit IgG highly cross adsorbed antibody used at a dilution of 1/100.
  • ab84697, at a dilution of 1/500, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
    Detection: DAB staining
  • ab84697, at a dilution of 1/500, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
    Detection: DAB staining
  • ab84697, at a dilution of 1/100, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
    Detection: DAB staining

Anti-CENPF antibody (ab84697)参考文献

ab84697 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Jul 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"