Anti-CENPF抗体(ab84697)
Key features and details
- Rabbit polyclonal to CENPF
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CENPF抗体
参阅全部 CENPF 一抗 -
描述
兔多克隆抗体to CENPF -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide:
QAEIQIKEES KTAVEMLQN Q LKELNEAV AA LCGDQEI MKA TEQSLD PPIE EEHQL RNSIE
, corresponding to amino acids 2350-2400 of Human CENPF -
阳性对照
- IHC: human ewing sarcoma
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab84697 was affinity purified using an epitope specific to CENPF immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84697于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. -
疾病相关
Stromme syndrome -
序列相似性
Belongs to the centromere protein F family. -
发展阶段
Gradually accumulates during the cell cycle, reaching peak levels in G2 and M phase, and is rapidly degraded upon completion of mitosis. -
翻译后修饰
Hyperphosphorylated during mitosis. -
细胞定位
Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body. - Information by UniProt
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数据库链接
- Entrez Gene: 1063 Human
- Omim: 600236 Human
- SwissProt: P49454 Human
- Unigene: 497741 Human
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别名
- AH antigen antibody
- Cell cycle dependent 350K nuclear protein antibody
- CENF antibody
see all
图片
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ab84697, at a dilution of 1/100, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
Detection: Red fluorescent goat anti-rabbit IgG highly cross adsorbed antibody used at a dilution of 1/100. -
ab84697, at a dilution of 1/500, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
Detection: DAB staining -
ab84697, at a dilution of 1/500, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
Detection: DAB staining -
ab84697, at a dilution of 1/100, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
Detection: DAB staining
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab84697 被引用在 1 文献中.
- Han Y et al. CENPF promotes papillary thyroid cancer progression by mediating cell proliferation and apoptosis. Exp Ther Med 21:401 (2021). PubMed: 33680123