使用敲除细胞株进行验证RabMAb

Anti-CENPE抗体[EPR4543(2)] (ab124733)

概述

  • 产品名称Anti-CENPE抗体[EPR4543(2)]
    参阅全部 CENPE 一抗
  • 描述
    兔单克隆抗体[EPR4543(2)] to CENPE
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human CENPE.

  • 阳性对照
    • HepG2, HeLa, and Jurkat cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab124733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 320 kDa (predicted molecular weight: 316 kDa).
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • 靶标

    • 相关性CENPE is a 250-300 kDa human centromere-associated kinesin-like motor protein that accumulates in G2 phase. In contrast to other centromere proteins, CENPE is not detected at centromeres during interphase, and first appears at the centromere region of chromosomes during prometaphase. CENPE function is required for the transition from metaphase to anaphase. CENPE is probably one of the motors responsible for mammalian chromosome movement and/or spindle elongation.
    • 细胞定位Associates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.
    • 数据库链接
    • 别名
      • CENP E antibody
      • Centromere associated protein E antibody
      • Centromere autoantigen E (312kD) antibody
      • Centromere autoantigen E antibody
      • Centromere protein E 312kDa antibody
      • Centromere protein E antibody
      • KIF10 antibody
      • Kinesin family member 10 antibody
      • Kinesin related protein antibody
      • Kinesin related protein CENPE antibody
      • PPP1R61 antibody
      • Protein phosphatase 1, regulatory subunit 61 antibody
      see all

    Anti-CENPE antibody [EPR4543(2)] 图像



    • Predicted band size : 316 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CENPE knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab124733 observed at 310 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab124733 was shown to specifically react with CENPE when CENPE knockout samples were used. Wild-type and CENPE knockout samples were subjected to SDS-PAGE. ab124733 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-CENPE antibody [EPR4543(2)] (ab124733) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 316 kDa

    Anti-CENPE antibody [EPR4543(2)] (ab124733)参考文献

    ab124733 has not yet been referenced specifically in any publications.

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