概述

  • 产品名称Anti-CENPC抗体
    参阅全部 CENPC 一抗
  • 描述
    兔多克隆抗体to CENPC
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 700 - 800 of Human CENPC.

    (Peptide available as ab33339.)

  • 阳性对照
    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate and Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab33034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 107 kDa).

靶标

  • 功能Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions.
  • 序列相似性Belongs to the CENPC family.
  • 结构域The MIF2 homology domain II targets centromeres and binds the alpha satellite DNA in vivo. The MIF2 homology domain III can induce CENPC dimerization/oligomerization.
  • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CENP-C 1 antibody
    • CENP-C antibody
    • CENPC_HUMAN antibody
    • CENPC1 antibody
    • Centromere autoantigen C antibody
    • Centromere autoantigen C1 antibody
    • Centromere protein C 1 antibody
    • Centromere protein C antibody
    • hcp-4 antibody
    • ICEN7 antibody
    • Interphase centromere complex protein 7 antibody
    • MIF2 antibody
    see all

Anti-CENPC antibody 图像

  • All lanes : Anti-CENPC antibody (ab33034) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 2 : Jurkat whole cell lysate (ab7899) at 20 µg

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 107 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab33034 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33034, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • ab33034 (1/200) staining CENPC in HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

Anti-CENPC antibody (ab33034)参考文献

This product has been referenced in:
  • Eliezer Y  et al. Interplay between the DNA damage proteins MDC1 and ATM in the regulation of the spindle assembly checkpoint. J Biol Chem 289:8182-93 (2014). WB . Read more (PubMed: 24509855) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Lymphoblast chromosomes)
Specification Lymphoblast chromosomes
Fixative Carnoy (Methanlo:Acetic Acid 3:1)
Permeabilization Yes - 0,25% Triton X-100 in blocking buffer
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Miss. Marie-Chantal Grégoire

Verified customer

提交于 Sep 30 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Username

Dr. Kirk McManus

Verified customer

提交于 Mar 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"