Anti-CENPC抗体[2159C5a] (ab50974)
Key features and details
- Mouse monoclonal [2159C5a] to CENPC
- Suitable for: WB, Dot blot, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-CENPC抗体[2159C5a]
参阅全部 CENPC 一抗 -
描述
小鼠单克隆抗体[2159C5a] to CENPC -
宿主
Mouse -
经测试应用
适用于: WB, Dot blot, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human CENPC aa 655-768.
Sequence:KPQTSGYTCNIPTESNLDSGEHKTSVLEESGPSRLNNNYLMSGKNDVDDE EVHGSSDDSKQSKVIPKNRIHHKLVLPSNTPNVRRTKRTRLKPLEYWRGE RIDYQGRPSGGFVI
Database link: Q03188 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
2159C5a -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50974于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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ICC/IF | (3) |
Use at an assay dependent concentration.
Use at a dilution of 1/250 (for overnight incubation). |
说明 |
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WB
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. Use at a dilution of 1/250 (for overnight incubation). |
靶标
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功能
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions. -
序列相似性
Belongs to the CENPC family. -
结构域
The MIF2 homology domain II targets centromeres and binds the alpha satellite DNA in vivo. The MIF2 homology domain III can induce CENPC dimerization/oligomerization. -
细胞定位
Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. - Information by UniProt
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数据库链接
- Entrez Gene: 1060 Human
- Omim: 117141 Human
- SwissProt: Q03188 Human
- Unigene: 479867 Human
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别名
- CENP-C 1 antibody
- CENP-C antibody
- CENPC_HUMAN antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (16)
ab50974 被引用在 16 文献中.
- Chou HC et al. The human origin recognition complex is essential for pre-RC assembly, mitosis, and maintenance of nuclear structure. Elife 10:N/A (2021). PubMed: 33522487
- Chan DY et al. Multigene human artificial chromosome vector delivery with herpes simplex virus 1 amplicons. Exp Cell Res 388:111840 (2020). PubMed: 31930965
- Zielinska AP et al. Meiotic Kinetochores Fragment into Multiple Lobes upon Cohesin Loss in Aging Eggs. Curr Biol 29:3749-3765.e7 (2019). PubMed: 31679939
- Nomani A et al. Evaluation of genotoxicity and mutagenic effects of vector/DNA nanocomplexes in transfected mesenchymal stem cells by flow cytometry. Acta Biomater 74:236-246 (2018). PubMed: 29783088
- Platani M et al. Seh1 targets GATOR2 and Nup153 to mitotic chromosomes. J Cell Sci 131:N/A (2018). PubMed: 29618633