- 数据表
- 具体的参考文献 (23)
- 实验方案
概述
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产品名称Anti-CENPB抗体
参阅全部 CENPB 一抗 -
描述兔多克隆抗体to CENPB
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宿主Rabbit
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经测试应用适用于: WB, IHC-P, ICC/IFmore details
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种属反应性与反应: Mouse, Rat, Human
预测可用于: Sheep, Hamster
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human CENPB.
(Peptide available as ab28019.)
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阳性对照
- This antibody gave a positive signal in the following: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Testis (Mouse) Tissue Lysate - normal tissue Ovary (Mouse) Tissue Lysate - normal tissue PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab25734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IHC-P | ||
| ICC/IF |
IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.
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序列相似性Contains 1 HTH CENPB-type DNA-binding domain.
Contains 1 HTH psq-type DNA-binding domain. -
细胞定位Nucleus. Chromosome > centromere.
- Information by UniProt
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数据库链接
- Entrez Gene: 1059 Human
- Entrez Gene: 12616 Mouse
- Entrez Gene: 362217 Rat
- Omim: 117140 Human
- SwissProt: P07199 Human
- SwissProt: P27790 Mouse
- SwissProt: P49451 Sheep
- Unigene: 516855 Human
see all -
别名
- CENP B antibody
- CENP-B antibody
- CENPB antibody
see all
图片
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Western blot - Anti-CENPB antibody (ab25734)All lanes : Anti-CENPB antibody (ab25734) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 74 kDa (possible cross reactivity), 75 kDa (possible cross reactivity) -
Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab25734)Image courtesy of an AbReview by Weiguo Zhang4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CENPB (red) using ab25734 at 1/200 dilution in ICC/IF.
The nuclear counterstain is DAPI (blue). Alpha-Tubulin is stained with an mouse monoclonal anti-alpha Tubulin antibody (green).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPB antibody (ab25734)Image courtesy of Human Protein Atlas. ab25734 staining CENPB in Human skin, showing nuclear staining in the epidermis and dermal layer. Paraffin embedded human skin tissue was incubated with ab25734(1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab25734 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
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Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab25734)This image is courtesy of an Abreview submitted by Dr Kirk McManusab25734 (1/1000) staining CENPB in HeLa cells (green). Cells were fixed with methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details. -
Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab25734)ICC/IF image of ab25734 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab25734, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293 cells.
实验方案
文献
This product has been referenced in:
- Thakur J & Henikoff S Unexpected conformational variations of the human centromeric chromatin complex. Genes Dev 32:20-25 (2018). Read more (PubMed: 29386331) »
- Tiwari A et al. 53BP1 can limit sister-chromatid rupture and rearrangements driven by a distinct ultrafine DNA bridging-breakage process. Nat Commun 9:677 (2018). Read more (PubMed: 29445165) »
客户评价及客户问答
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