Anti-CENPA (phospho S7)抗体(ab62242)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-CENPA (phospho S7)抗体
参阅全部 CENPA 一抗 -
描述兔多克隆抗体to CENPA (phospho S7)
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宿主Rabbit
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特异性ab62242 detects endogenous levels of CENPA only when phosphorylated at serine 7.
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经测试应用适用于: ELISA, ICC/IFmore details
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种属反应性与反应: Human
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免疫原
Synthetic phosphopeptide derived from human CENPA around the phosphorylation site of serine 7 (R-R-SP-R-K).
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阳性对照
- HeLa cells.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明ab62242 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab62242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/10000. | |
| ICC/IF | 1/500 - 1/1000. |
靶标
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功能Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
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序列相似性Belongs to the histone H3 family.
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结构域The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.
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翻译后修饰Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12. -
细胞定位Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.
- Information by UniProt
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数据库链接
- Entrez Gene: 1058 Human
- Omim: 117139 Human
- SwissProt: P49450 Human
- Unigene: 1594 Human
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别名
- CENP A antibody
- CENP-A antibody
- cenpa antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CENPA (phospho S7) antibody (ab62242)This image is courtesy of an abreview by Kirk Mcmanusab62242 staining CENPA (phospho S7) in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Methanol. Samples were incubated with primary antibody (1/400 in PBS) for 1 hour at 22°C. Undiluted ab150081 was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-CENPA (phospho S7) antibody (ab62242)ab62242 at 1/500 - 1/1000 dilution staining CENPA in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
文献
This product has been referenced in:
- Santaguida S et al. Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine. J Cell Biol 190:73-87 (2010). WB, ICC/IF ; Human . Read more (PubMed: 20624901) »
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