Anti-CENPA (phospho S7)抗体(ab62242)

概述

• 产品名称
  Anti-CENPA (phospho S7)抗体
  参阅全部 CENPA 一抗
• 描述
  兔多克隆抗体to CENPA (phospho S7)
• 特异性
  ab62242 detects endogenous levels of CENPA only when phosphorylated at serine 7.
• 经测试应用
  适用于: ELISA, ICC/IFmore details
• 种属反应性
  与反应: Human
  
• 免疫原

  Synthetic phosphopeptide derived from human CENPA around the phosphorylation site of serine 7 (R-R-S^P-R-K).

• 阳性对照
  • HeLa cells.

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
• 存储溶液
  Preservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• 纯度
  Immunogen affinity purified
• 纯化说明
  ab62242 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• 克隆
  多克隆
• 同种型
  IgG
• 研究领域
  • Epigenetics and Nuclear Signaling
  • Histones
  • Variants

  • Epigenetics and Nuclear Signaling
  • Chromosome Structure
  • Centromeres

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Chromosome Structure
  • Centromere

相关产品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

应用

靶标

• 功能
  Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
• 序列相似性
  Belongs to the histone H3 family.
• 结构域
  The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.
• 翻译后修饰
  Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
  Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.
• 细胞定位
  Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.

• Target information above from: UniProt accession P49450 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 1058 Human
  • Omim: 117139 Human
  • SwissProt: P49450 Human
  • Unigene: 1594 Human
• 别名
  • CENP A antibody
  • CENP-A antibody
  • cenpa antibody

  • CENPA_HUMAN antibody
  • Centromere autoantigen A antibody
  • Centromere protein A 17kDa antibody
  • Centromere protein A antibody
  • Histone H3 like centromeric protein A antibody
  • Histone H3-like centromeric protein A antibody

  see all

实验方案

• Immunocytochemistry & immunofluorescence protocols