概述

  • 产品名称Anti-CENPA抗体
    参阅全部 CENPA 一抗
  • 描述
    兔多克隆抗体to CENPA
  • 特异性Detects endogenous levels of total CENPA protein.
  • 经测试应用适用于: ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human CENPA around the phosphorylation site of serine 7 (R-R-SP-R-K).

性能

应用

Our Abpromise guarantee covers the use of ab62688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
  • 序列相似性Belongs to the histone H3 family.
  • 结构域The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.
  • 翻译后修饰Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
    Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.
  • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CENP A antibody
    • CENP-A antibody
    • cenpa antibody
    • CENPA_HUMAN antibody
    • Centromere autoantigen A antibody
    • Centromere protein A 17kDa antibody
    • Centromere protein A antibody
    • Histone H3 like centromeric protein A antibody
    • Histone H3-like centromeric protein A antibody
    see all

Anti-CENPA antibody 图像

  • ab62688 at 1/500 - 1/1000 dilution staining CENPA in HeLa cells (treated with Forskolin (40nM, 30mins) or untreated) by Immunofluorescence,

Anti-CENPA antibody (ab62688)参考文献

ab62688 has not yet been referenced specifically in any publications.

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