Anti-Cenexin1/ODF2抗体(ab43840)

概述

  • 产品名称Anti-Cenexin1/ODF2抗体
    参阅全部 Cenexin1/ODF2 一抗
  • 描述
    兔多克隆抗体to Cenexin1/ODF2
  • 特异性On the basis of the immunogen sequence, we would expect this antibody to recognise the following isoforms of Cenexin1/ODF2 as listed in Swissprot: Q5BJF6-1 (95 kDa), Q5BJF6-2 (89 kDa), Q5BJF6-3 (93 kDa), and Q5BJF6-4 (95 kDa).
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human Cenexin1/ODF2.

    (Peptide available as ab43839.)

  • 阳性对照
    • This antibody gave a positive signal in Hela whole cell lysate and Human testis tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab43840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 22096071

靶标

  • 功能Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.
  • 组织特异性Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails.
  • 序列相似性Belongs to the ODF2 family.
  • 翻译后修饰Tyrosine phosphorylated.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome. Cell projection > cilium. Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 84 kDa outer dense fiber protein antibody
    • Cancer/testis antigen 134 antibody
    • Cenexin 1 antibody
    • Cenexin antibody
    • Cenexin1 variant 1 antibody
    • CT134 antibody
    • FLJ44866 antibody
    • KKT 4 antibody
    • KKT4 antibody
    • MGC111096 antibody
    • MGC9034 antibody
    • ODF 2 antibody
    • ODF 84 antibody
    • odf2 antibody
    • ODF2/1 antibody
    • ODF2/2 antibody
    • ODF84 antibody
    • ODFP2_HUMAN antibody
    • OTTHUMP00000022274 antibody
    • OTTHUMP00000022275 antibody
    • Outer dense fiber of sperm tail 2 antibody
    • Outer dense fiber of sperm tails 2 antibody
    • Outer dense fiber of sperm tails protein 2 antibody
    • Outer dense fiber of sperm tails, 84 kD antibody
    • Outer dense fiber protein 2 antibody
    • Sperm outer dense fiber major protein 2 antibody
    • Sperm tail structural protein antibody
    • Testis specific autoantigen antibody
    see all

Anti-Cenexin1/ODF2 antibody 图像

  • ab43840 staining Cenexin1 in the mother centriole of a dividing HeLa cell by ICC/IF. DNA is stained blue, Aurora A is stained red, and Cenexin1 is stained green. Cells were fixed in formaldehyde, permeabilized with 0.1% Triton in PBS, and blocked with 2% BSA for 1 hour at 21°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 21°C. A FITC-conjugated anti-rabbit IgG H+L antibody was used as the secondary.

    See Abreview

  • All lanes : Anti-Cenexin1/ODF2 antibody (ab43840) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 25 µg
    Lane 2 : Human testis tissue lysate - total protein (ab30257) at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • All lanes : Anti-Cenexin1/ODF2 antibody (ab43840) at 1/500 dilution

    Lane 1 : 25 ug HeLa cell lysate
    Lane 2 : 12.5 ug HeLa cell lysate
    Lane 3 : 25 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (ab43839) at 4 µg/ml
    Lane 4 : 12.5 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (ab43839) at 4 µg/ml

    Secondary
    Anti-Rabbit IgG HRP-conjugated at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : ~75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    Image courtesy of an anonymous abreview.

    See Abreview

Anti-Cenexin1/ODF2 antibody (ab43840)参考文献

This product has been referenced in:
  • Herawati E  et al. Multiciliated cell basal bodies align in stereotypical patterns coordinated by the apical cytoskeleton. J Cell Biol 214:571-86 (2016). Read more (PubMed: 27573463) »
  • Dantas TJ  et al. Calcium-binding capacity of centrin2 is required for linear POC5 assembly but not for nucleotide excision repair. PLoS One 8:e68487 (2013). ICC/IF . Read more (PubMed: 23844208) »

See all 7 Publications for this product

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - PBS, 0.1% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Apr 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 25 µg
Specification HeLa
Treatment 4 ug/ml immunogen peptide
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

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提交于 Apr 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"