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RabMAb

Anti-CEND1抗体[EPR3739] (ab113076)

概述

  • 产品名称
    Anti-CEND1抗体[EPR3739]
    参阅全部 CEND1 一抗
  • 描述
    兔单克隆抗体[EPR3739] to CEND1
  • 经测试应用
    适用于: WB, IHC-P, ICC, IP, IHC-Frmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human CEND1.

  • 阳性对照
    • Human fetal brain lysate, U-87 MG and Neuro 2a cell lysates; Human brain glioma tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab113076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min
ICC 1/250 - 1/500.
IP 1/50.
IHC-Fr Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    图片

    • All lanes : Anti-CEND1 antibody [EPR3739] (ab113076) at 1/10000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : U-87 MG cell lysate
      Lane 3 : Neuro 2a cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 15 kDa
    • ab113076 at 1/500 dilution, staining CEND1 in Praffin-embedded Human brain tissue by Immunohistochemistry.
    • ab113076 at 1/500 dilution, staining CEND1 in Praffin-embedded Human glioma tissue by Immunohistochemistry.
    • ab113076 staining CEND1 in Rat retina (at birth) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000) for 24 hours. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    文献

    ab113076 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample
    Rat Tissue sections (retina of rat at birth)
    Specification
    retina of rat at birth
    Permeabilization
    Yes - 0.1% Triton
    Fixative
    Formaldehyde
    Username

    Mrs. Claudine Botteri

    Verified customer

    提交于 Feb 25 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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