The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IHC-P: Ready to use for 10 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
组织特异性Highly expressed in proliferating cells.
序列相似性Belongs to the XPO2/CSE1 family. Contains 1 importin N-terminal domain.
细胞定位Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.