Anti-Cellular Apoptosis Susceptibility抗体(ab70547)

概述

  • 产品名称Anti-Cellular Apoptosis Susceptibility抗体
    参阅全部 Cellular Apoptosis Susceptibility 一抗
  • 描述
    兔多克隆抗体to Cellular Apoptosis Susceptibility
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide mapping to a region between residues 1 and 50 of human Cellular Apoptosis Susceptibility using the numbering given in entry NP_001307.2

  • 阳性对照
    • Whole cell lysate from HeLa and Ramos cells

性能

应用

Our Abpromise guarantee covers the use of ab70547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/25000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
  • 组织特异性Highly expressed in proliferating cells.
  • 序列相似性Belongs to the XPO2/CSE1 family.
    Contains 1 importin N-terminal domain.
  • 细胞定位Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAS antibody
    • Cellular apoptosis susceptibility protein antibody
    • Chromosome segregation 1 (yeast homolog) like antibody
    • Chromosome segregation 1 Like antibody
    • Chromosome segregation 1 like protein antibody
    • Chromosome segregation 1-like protein antibody
    • Chromosome segregation gene CSE1 antibody
    • CSE 1 antibody
    • CSE 1 chromosome segregation 1 like antibody
    • CSE 1 chromosome segregation 1 like protein antibody
    • CSE 1L antibody
    • CSE1 antibody
    • CSE1 chromosome segregation 1 like (yeast) antibody
    • CSE1 chromosome segregation 1 like antibody
    • CSE1 chromosome segregation 1 like protein antibody
    • CSE1L antibody
    • Exp 2 antibody
    • Exp2 antibody
    • Exportin 2 antibody
    • Exportin-2 antibody
    • Exportin2 antibody
    • Importin alpha re exporter antibody
    • Importin-alpha re-exporter antibody
    • MGC117283 antibody
    • MGC130036 antibody
    • MGC130037 antibody
    • XPO 2 antibody
    • XPO2 antibody
    • XPO2_HUMAN antibody
    see all

Anti-Cellular Apoptosis Susceptibility antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node (left) and mouse squamous cell carcinoma (right) labelling Cellular Apoptosis Susceptibility with ab70547 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Cellular Apoptosis Susceptibility antibody (ab70547) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from Ramos cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 110 kDa
    Observed band size : 110 kDa


    Exposure time : 3 minutes
  • 1mg whole cell lysate was immunoprecipitated using ab70547 at 3ug/ml (lane 1) or a control IgG (lane 2). For the subsequent blot 25% of the immunoprecipitate was loaded per lane and probed with ab70547 at 1ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.

Anti-Cellular Apoptosis Susceptibility antibody (ab70547)参考文献

ab70547 has not yet been referenced specifically in any publications.

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