The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/10000 - 1/25000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
Use at 2-5 µg/mg of lysate.
Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Highly expressed in proliferating cells.
Belongs to the XPO2/CSE1 family. Contains 1 importin N-terminal domain.
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node (left) and mouse squamous cell carcinoma (right) labelling Cellular Apoptosis Susceptibility with ab70547 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-Cellular Apoptosis Susceptibility antibody (ab70547)
All lanes : Anti-Cellular Apoptosis Susceptibility antibody (ab70547) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from Ramos cells at 50 µg
Developed using the ECL technique.
Predicted band size: 110 kDa Observed band size: 110 kDa
1mg whole cell lysate was immunoprecipitated using ab70547 at 3ug/ml (lane 1) or a control IgG (lane 2). For the subsequent blot 25% of the immunoprecipitate was loaded per lane and probed with ab70547 at 1ug/ml. Detection:
chemiluminescence with an exposure time of 3 minutes.