概述

  • 产品名称Anti-Cellubrevin抗体
    参阅全部 Cellubrevin 一抗
  • 描述
    兔多克隆抗体to Cellubrevin
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Mouse Cellubrevin aa 1-18.
    Sequence:

    MSTGVPSGSSAATGSNRR


    (Peptide available as ab5861)

  • 阳性对照
    • In Western Blot HeLa cell extract (ab7898) can be used as a positive control.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, all VAMP isoform levels appear to be significantly reduced.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).

靶标

Anti-Cellubrevin antibody 图像



  • Predicted band size : 12 kDa

    Western blot analysis of recombinant rat VAMP-3 using ab5789.



  • Predicted band size : 12 kDa

    Western blot analysis of VAMP-3 in HeLa cell extract using ab5789.

Anti-Cellubrevin antibody (ab5789)参考文献

This product has been referenced in:
  • Caceres PS  et al. Vesicle-associated membrane protein 2 (VAMP2) but Not VAMP3 mediates cAMP-stimulated trafficking of the renal Na+-K+-2Cl- co-transporter NKCC2 in thick ascending limbs. J Biol Chem 289:23951-62 (2014). WB ; Rat . Read more (PubMed: 25008321) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing (4-20 % gradient gel)
Sample Human Cell lysate - whole cell (HeLa, U266)
Specification HeLa, U266
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 13 2013

Thank you for your note. I am sorry to hear that even the replacement vial failed to work. This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab68833. For you...

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Thank you for your response and for reattaching the image. I can arrange the credit note for the cost of ab68833, our Abpromise applies for this complaint. Initially, the problem was reported only with ab68833 and looking at our system there is no reco...

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Thank you for your new contact and updates. Unfortunately, one the files you have kindly sent to us can’t be opened so I am afraid I am unable to take a look at it. Could you resend it to me? According to our last correspondence (16th December 20...

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Thank you for your email. Ab5789 was characterized for use in Western blotting and was not tested in other applications. By Western blot, the antibody detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa) representing recombinant ...

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Thank you for your enquiry. Ab5789 has not been tested for cross-reactivity with the other VAMP isoforms. However, if you BLAST the peptide sequence used to generate this antibody (the ExPASy and NCBI links are on the online datasheet) there is no iden...

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Regarding ab2102 and ab2103, unfortunately the peptide sequences are not available. If you have any additional questions, please let us know.

Thank you for your enquiry. Cellubrevin is an alternative name for VAMP3, and so these 3 antibodies target the same protein. Ab2103 has been tested for cross-reactivity with Human and Mouse, and does not cross-react with Rat or Dog. Ab2102 cro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"