Anti-Cellubrevin抗体(ab5789)
Key features and details
- Rabbit polyclonal to Cellubrevin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cellubrevin抗体
参阅全部 Cellubrevin 一抗 -
描述
兔多克隆抗体to Cellubrevin -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Mouse Cellubrevin aa 1-18.
Sequence:MSTGVPSGSSAATGSNRR
(Peptide available asab5861) -
阳性对照
- WB: HeLa cell extract (ab7898) ; Mouse Brain ; Rat Brain ICC/IF: NIH/3T3 (Mouse embryonic fibroblast cell line) ; Neuro-2a (Mouse neuroblastoma cell line)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
Primary antibody说明
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, all VAMP isoform levels appear to be significantly reduced. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/500. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
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ICC/IF |
1/50.
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说明 |
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WB
1/500. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa). |
ICC/IF
1/50. |
靶标
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功能
SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. -
序列相似性
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Membrane. Cell junction > synapse > synaptosome. - Information by UniProt
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数据库链接
- Entrez Gene: 9341 Human
- Entrez Gene: 22319 Mouse
- Entrez Gene: 29528 Rat
- Omim: 603657 Human
- SwissProt: Q15836 Human
- SwissProt: P63024 Mouse
- SwissProt: P63025 Rat
- Unigene: 66708 Human
see all -
别名
- CEB antibody
- Cellubrevin antibody
- Synaptobrevin 3 antibody
see all
图片
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All lanes : Anti-Cellubrevin antibody (ab5789)
Lane 1 : Mouse Brain
Lane 2 : Rat Brain
Lane 3 : Mouse Liver
Lane 4 : Rat Liver
Predicted band size: 12 kDa
Additional bands at: ~15 kDa. We are unsure as to the identity of these extra bands.Relative expression of Cellubrevin observed in Mouse Brain and Rat Brain in comparison to Mouse Liver and Rat Liver using ab5789 to demonstrate specificty of expression of target across different tissues.
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Western blot analysis of recombinant rat VAMP-3 using ab5789.
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Western blot analysis of VAMP-3 in HeLa cell extract using ab5789.
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Immunofluorescent analysis of formalin-fixed, 0.1% Triton™ X-100 permeabilized, NIH/3T3 (Mouse embryonic fibroblast cell line) cells, blocked with 3% BSA-PBS for 30 minutes at room temperature. Labelling cellubrevin with ab5789 at 1/50 dilution and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody at room temperature in the dark (right image). Compared with negative control without primary antibody (left image). Nuclei were stained with DAPI (Blue). Actin was stained with Alexa Fluor® 555 (Red). The images were captured at 60X magnification.
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Immunofluorescent analysis of formalin-fixed, 0.1% Triton™ X-100 permeabilized, Neuro-2a (Mouse neuroblastoma cell line) cells, blocked with 3% BSA-PBS for 30 minutes at room temperature. Labelling cellubrevin with ab5789 at 1/50 dilution and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody at room temperature in the dark (right image). Compared with negative control without primary antibody (left image). Nuclei were stained with DAPI (Blue).The images were captured at 60X magnification.
数据表及文件
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Datasheet download
文献 (5)
ab5789 被引用在 5 文献中.
- Lasic E et al. Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action. Sci Rep 9:10957 (2019). PubMed: 31358895
- Habtemichael EN et al. Usp25m protease regulates ubiquitin-like processing of TUG proteins to control GLUT4 glucose transporter translocation in adipocytes. J Biol Chem 293:10466-10486 (2018). PubMed: 29773651
- Verboogen DRJ et al. Secretory vesicles of immune cells contain only a limited number of interleukin 6 molecules. FEBS Lett 592:1535-1544 (2018). PubMed: 29570778
- Verboogen DRJ et al. Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation. Elife 6:N/A (2017). WB, ICC/IF ; Human . PubMed: 28524818
- Caceres PS et al. Vesicle-associated membrane protein 2 (VAMP2) but Not VAMP3 mediates cAMP-stimulated trafficking of the renal Na+-K+-2Cl- co-transporter NKCC2 in thick ascending limbs. J Biol Chem 289:23951-62 (2014). WB ; Rat . PubMed: 25008321